Mouse anti-Human UNC45A Polyclonal Antibody | anti-UNC45A antibody

UNC45A (Protein Unc-45 Homolog A, Unc-45A, GCUNC-45, Smooth Muscle Cell-associated Protein 1, SMAP1, SMAP-1)

Cat. Num. AAA648137

• Gene Names: UNC45A; SMAP1; SMAP-1; GCUNC45; GC-UNC45; GCUNC-45; IRO039700
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: UNC45A; Polyclonal Antibody; UNC45A (Protein Unc-45 Homolog A; Unc-45A; GCUNC-45; Smooth Muscle Cell-associated Protein 1; SMAP1; SMAP-1); Anti -UNC45A (Protein Unc-45 Homolog A; anti-UNC45A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UNC45A.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MTVSGPGTPEPRPATPGASSVEQLRKEGNELFKCGDYGGALAAYTQALGLDATPQDQAVLHRNRAACHLKLEDYDKAETEASKAIEKDGGDVKALYRRSQALEKLGRLDQAVLDLQRCVSLEPKNKVFQEALRNIGGQIQEKVRYMSSTDAKVEQMFQILLDPEEKGTEKKQKASQNLVVLAREDAGAEKIFRSNGVQLLQRLLDMGETDLMLAALRTLVGICSEHQSRTVATLSILGTRRVVSILGVESQAVSLAACHLLQVMFDALKEGVKKGFRGKEGAIIVDPARELKVLISNLLDLLTEVGVSGQGRDNALTLLIKAVPRKSLKDPNNSLTLWVIDQGLKKILEVGGSLQDPPGELAVTANSRMSASILLSKLFDDLKCDAERENFHRLCENYIKSWFEGQGLAGKLRAIQTVSCLLQGPCDAGNRALELSGVMESVIALCASEQEEEQLVAVEALIHAAGKAKRASFITANGVSLLKDLYKCSEKDSIRIRALVGLCKLGSAGGTDFSMKQFAEGSTLKLAKQCRKWLCNDQIDAGTRRWAVEGLAYLTFDADVKEEFVEDAAALKALFQLSRLEERSVLFAVASALVNCTNSYDYEEPDPKMVELAKYAKQHVPEQHPKDKPSFVRARVKKLLAAGVVSAMVCMVKTESPVLTSSCRELLSRVFLALVEEVEDRGTVVAQGGGRALIPLALEGTDVGQTKAAQALAKLTITSNPEMTFPGERIYEVVRPLVSLLHLNCSGLQNFEALMALTNLAGISERLRQKILKEKAVPMIEGYMFEEHEMIRRAATECMCNLAMSKEVQDLFEAQGNDRLKLLVLYSGEDDELLQRAAAGGLAMLTSMRPTLCSRIPQVTTHWLEILQALLLSSNQELQHRGAVVVLNMVEASREIASTLMESEMMEILSVLAKGDHSPVTRAAAACLDKAVEYGLIQPNQDGE

• Applicable Applications for anti-UNC45A antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human UNC45A, aa1-944 (NP_061141).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UNC45A polyclonal antibody. Western Blot analysis of UNC45A expression in human kidney.)

Western Blot (WB)

(Western Blot analysis of UNC45A expression in transfected 293T cell line by UNC45A polyclonal antibody. Lane 1: UNC45A transfected lysate (103.84kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to UNC45A on HeLa cell. [antibody concentration 10ug/ml].)

Product Categories/Family for anti-UNC45A antibody

Antibodies; Abs to Hormones; Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 39644901
• NCBI GeneID: 55898
• UniProt Accession #: Q9H3U1; Q7L3Y6; Q9H3U8; Q9NSE8; Q9NSE9; A8K6F7
• Molecular Weight: 103,077 Da
• NCBI Official Full Name: UNC45A protein, partial
• NCBI Official Synonym Full Names: unc-45 homolog A (C. elegans)
• NCBI Official Symbol: UNC45A
• NCBI Official Synonym Symbols: SMAP1; SMAP-1; GCUNC45; GC-UNC45; GCUNC-45; IRO039700
• NCBI Protein Information: protein unc-45 homolog A; UNC-45A; general cell UNC45; smooth muscle cell associated protein-1; smooth muscle cell-associated protein 1
• UniProt Protein Name: Protein unc-45 homolog A
• Protein Family: Protein
• UniProt Gene Name: UNC45A
• UniProt Synonym Gene Names: SMAP1; Unc-45A; SMAP-1
• UniProt Entry Name: UN45A_HUMAN

NCBI Description

UNC45A plays a role in cell proliferation and myoblast fusion, binds progesterone receptor (PGR; MIM 607311) and HSP90 (HSPCA; MIM 140571), and acts as a regulator of the progesterone receptor chaperoning pathway (Price et al., 2002 [PubMed 12356907]; Chadli et al., 2006 [PubMed 16478993]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Acts as co-chaperone for HSP90. Prevents the stimulation of HSP90AB1 ATPase activity by AHSA1. Positive factor in promoting PGR function in the cell. May be necessary for proper folding of myosin

Potential. Necessary for normal cell proliferation. Necessary for normal myotube formation and myosin accumulation during muscle cell development. May play a role in erythropoiesis in stroma cells in the spleen

By similarity. Ref.6 Ref.13

Subunit structure: Interacts with PGR isoforms A and B as well as with NR3C1 in the absence of ligand, and with HSP90AB1. Binding to HSP90AB1 involves 2 UNC45A monomers per HSP90AB1 dimer. Ref.13

Subcellular location: Cytoplasm. Cytoplasm › perinuclear region. Nucleus. Note: Predominant in the perinuclear region. Little protein in the nucleus. Ref.13

Tissue specificity: Detected in peripheral blood leukocytes, bone marrow, adrenal gland, trachea, spinal cord, thyroid, lymph node and stomach. Ref.6

Sequence similarities: Contains 3 TPR repeats.