Rabbit anti-Mouse UCHL1 Monoclonal Antibody | anti-UCHL1 antibody

Recombinant Anti-UCHL1 Antibody, Rabbit Monoclonal

Cat. Num. AAA8111911

• Gene Names: Uchl1; gad; C88048; PGP9.5; R75593; UCH-L1; UCHL-1; PGP 9.5; AW822034
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A
• Synonyms: UCHL1; Monoclonal Antibody; Recombinant Anti-UCHL1 Antibody; Rabbit Monoclonal; UCHL1 Antibody; Rabbit MAb; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Anti-AW822034 Antibody; Anti-C88048 Antibody; Anti-gad Antibody; Anti-PGP9.5 Antibody; Anti-R75593 Antibody; Anti-UCH-L1 Antibody; Anti-UCHL-1 Antibody; anti-UCHL1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 011
• Specificity: Mouse UCHL1.; Has cross-reactivity in ELISA with Human UCHL1, Human UCHL3, Rat UCHL1; Has cross-reactivity in ELISA with Human UCHL1, Human UCHL3, Rat UCHL1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-UCHL1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 0.5-2uL/mg of lysate
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse UCHL1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse UCHL1 (rM UCHL1).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-UCHL1 rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:25 kDaObserved band size:27 kDa)

Immunoprecipitation (IP)

(Mouse UCHL1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg U87MG Whole Cell Lysate2 uL anti-Mouse UCHL1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse UCHL1 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size: 25 kDa)

Related Product Information for anti-UCHL1 antibody

Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.

References

Wada H., et al., 1998, Biochem. Biophys. Res. Commun. 251:688-92. Choi J., et al., 2004, J. Biol. Chem. 279:13256-64. Lombardino, A.2005, et al., J.Proc Natl Acad Sci.USA102 (22):8036-41 Okochi-Takada, E. et al., 2006, Int J Cancer. 119 (6):1338-44. Zetterberg, M. et al., 2010, Mol Neurodegener 5 :11.

NCBI and Uniprot Product Information

• NCBI GI #: 188219614
• NCBI GeneID: 22223
• NCBI Accession #: NP_035800.2
• NCBI GenBank Nucleotide #: NM_011670.2
• UniProt Accession #: Q9R0P9; Q9R122
• Molecular Weight: 24,838 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin carboxy-terminal hydrolase L1
• NCBI Official Symbol: Uchl1
• NCBI Official Synonym Symbols: gad; C88048; PGP9.5; R75593; UCH-L1; UCHL-1; PGP 9.5; AW822034
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: Uchl1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_MOUSE

Uniprot Description

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Cell development/differentiation; EC 3.4.19.12; Ligase; Protease; Ubiquitin conjugating system

Cellular Component: axon; cell soma; cytoplasm; cytosol; myelin sheath; nucleoplasm; plasma membrane

Molecular Function: alpha-2A adrenergic receptor binding; cysteine-type endopeptidase activity; omega peptidase activity; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity

Biological Process: adult walking behavior; axon target recognition; axon transport of mitochondrion; axonogenesis; cell proliferation; eating behavior; muscle fiber development; negative regulation of MAP kinase activity; neuromuscular process; protein deubiquitination; sensory perception of pain; ubiquitin-dependent protein catabolic process