Western Blot (WB)
(Western Blot analysis of in vitro linked and purified polyubiquitin chains UbK63 antibody. The antibody detects lysine-63-linked chains (Lane 1), but does not detect lysine-48-linked chains (Lane 2).)
Mouse UbK63 Monoclonal Antibody | anti-UbK63 antibody
UbK63 antibody
Applications
Flow Cytometry, Functional Assay, Functional Assay
Purity
UbK63 antibody was purified by affinity chromatography.
Synonyms
UbK63; Monoclonal Antibody; UbK63 antibody; Monoclonal UbK63; Anti-UbK63; UbK 63; Lysine63-linked poly ubiquitin chain; UbK-63; K63-linked polyubiquitin; anti-UbK63 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
HWA4C4
Specificity
Human
Purity/Purification
UbK63 antibody was purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-UbK63 antibody
Flow Cytometry (FC/FACS), Functional Assay
Application Notes
FC: <= 1 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
Biological Significance
Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms. Ubiquitin polymers can be found in several varieties depending on which of its 7 lysine residues is the site of ligation. The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 or lysine-63. K-48-linked polyubiquitination typically results in targeting the protein to the 26S proteosome resulting in proteolysis, although recently exceptions to this have been observed. In contrast, K-63-linked polyubiquitination results in signals related to intracellular trafficking, cell signaling, ribosomal biogenesis, and DNA damage repair. There are also reports that K63-linked polyubiquitin is involved in proteosome-independent proteolysis via autophagy.HWA4C4 reacts specifically with the isopeptide linkage region of lysine-63-linked polyubiquitin. It does not react with other forms of ubiquitin including ubiquitin monomer and lysine-48-linked chains.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Western Blot (WB)
(Western Blot analysis of in vitro linked and purified polyubiquitin chains UbK63 antibody. The antibody detects lysine-63-linked chains (Lane 1), but does not detect lysine-48-linked chains (Lane 2).)
Western Blot (WB)
(Western Blot analysis of in vitro linked and purified polyubiquitin chains UbK63 antibody. The antibody detects lysine-63-linked chains (Lane 1), but does not detect lysine-48-linked chains (Lane 2).)
Related Product Information for anti-UbK63 antibody
Mouse monoclonal UbK63 antibody
Product Categories/Family for anti-UbK63 antibody
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Product Notes
The UbK63 (Catalog #AAA832974) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's UbK63 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Functional Assay. FC: <= 1 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the UbK63 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UbK63, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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