Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with Rat CD44 antibodyfollowed by 0.015 ug of Mouse IgG1 kappa Isotype Control (left) or 0.015 ug of Anti-Allophycocyanin Purified (right) followed by detection with Anti-Mouse IgG (FITC). Total viable cells were used for analysis.)
Mouse Allophycocyanin Monoclonal Antibody | anti-apcB antibody
Allophycocyanin antibody
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Allophycocyanin antibody was purified by affinity chromatography.
Synonyms
Allophycocyanin; Monoclonal Antibody; Allophycocyanin antibody; Monoclonal Allophycocyanin; Anti-Allophycocyanin; APC; anti-apcB antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
APC-6A2
Purity/Purification
Allophycocyanin antibody was purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
161
Applicable Applications for anti-apcB antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
FC: <= 1ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
Biological Significance
The APC-6A2 monoclonal antibody reacts with allophycocyanin and APC-Cy7, with no crossreactivity to fluorescein or phycoerythrin. When using APC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated APC antibody. Therefore no interference or quenching of the APC fluorochrome is observed. The APC-6A2 can be used for separation of cells labeled with APC-conjugated antibodies or for staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary APC-conjugated antibody and the application tested.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with Rat CD44 antibodyfollowed by 0.015 ug of Mouse IgG1 kappa Isotype Control (left) or 0.015 ug of Anti-Allophycocyanin Purified (right) followed by detection with Anti-Mouse IgG (FITC). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with Rat CD44 antibodyfollowed by 0.015 ug of Mouse IgG1 kappa Isotype Control (left) or 0.015 ug of Anti-Allophycocyanin Purified (right) followed by detection with Anti-Mouse IgG (FITC). Total viable cells were used for analysis.)
Related Product Information for anti-apcB antibody
Mouse monoclonal Allophycocyanin antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
17,433 Da
NCBI Official Full Name
allophycocyanin (chromoplast)
UniProt Protein Name
Allophycocyanin beta chain
Protein Family
UniProt Gene Name
apcB
UniProt Entry Name
PHAB_GALSU
Uniprot Description
Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex. Allophycocyanin has a maximum absorption at approximately 650 nanometers.
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Product Notes
The Allophycocyanin apcb (Catalog #AAA833786) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Allophycocyanin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP). FC: <= 1ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the Allophycocyanin apcb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Allophycocyanin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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