Mouse Ubiquitin Conjugating Enzyme E2L 6 Monoclonal Antibody | anti-UBE2L6 antibody

Mouse Anti Human Ubiquitin Conjugating Enzyme E2L 6

Cat. Num. AAA140332

• Gene Names: UBE2L6; RIG-B; UBCH8
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: UBE2L6 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Ubiquitin Conjugating Enzyme E2L 6; Monoclonal Antibody; Mouse Anti Human Ubiquitin Conjugating Enzyme E2L 6; UBE2L6 Antibody; Mouse Anti Human; RIG-B; UBCH8; MGC40331; UBE2L6; Ubiquitin/ISG15-conjugating enzyme E2 L6; Ubiquitin-protein ligase L6; Ubiquitin carrier protein L6; Retinoic acid-induced gene B protein; anti-UBE2L6 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b heavy chain and kappa light chain
• Clone Number: Pk1H3AT
• Purity/Purification: UBE2L6 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 153

• Applicable Applications for anti-UBE2L6 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: UBE2L6 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:3,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:2,000 and Immunohistochemistry is 1:50.
• Immunogen: Anti-human UBE2L6 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human UBE2L6 amino acids 1-152 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-UBE2L6 antibody

Introduction: UBE2L6 belongs to the E2 ubiquitin-conjugating enzyme family. UBE2L6 enzyme is very similar in its primary structure to the enzyme encoded by UBE2L3 gene. UBE2L6 catalyzes the covalent attachment of ubiquitin to other proteins. UBE2L6 functions in the e6/e6-ap-induced ubiquitination of p53/tp53.

Product Categories/Family for anti-UBE2L6 antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 4759282
• NCBI GeneID: 9246
• NCBI Accession #: NP_004214.1
• NCBI GenBank Nucleotide #: NM_004223.4
• Molecular Weight: 10,086 Da
• NCBI Official Full Name: ubiquitin/ISG15-conjugating enzyme E2 L6 isoform 1
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2L 6
• NCBI Official Symbol: UBE2L6
• NCBI Official Synonym Symbols: RIG-B; UBCH8
• NCBI Protein Information: ubiquitin/ISG15-conjugating enzyme E2 L6; retinoic acid induced gene B protein; retinoic acid-induced gene B protein; ubiquitin carrier protein L6; ubiquitin-protein ligase L6
• UniProt Protein Name: Ubiquitin/ISG15-conjugating enzyme E2 L6
• Protein Family: Ubiquitin/ISG15-conjugating enzyme
• UniProt Gene Name: UBE2L6
• UniProt Synonym Gene Names: UBCH8; RIG-B
• UniProt Entry Name: UB2L6_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

UBE2L6: Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: cytosol

Molecular Function: ISG15 conjugating enzyme activity; protein binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: ISG15-protein conjugation; modification-dependent protein catabolic process; cytokine and chemokine mediated signaling pathway; innate immune response; protein ubiquitination; protein modification process; negative regulation of interferon type I production