CD146/MCAM Recombinant Protein | MCAM recombinant protein

CD146/MCAM Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120009

• Gene Names: MCAM; CD146; MUC18
• Purity: >98% as determined by SDS-PAGE
• Synonyms: CD146/MCAM; CD146/MCAM Protein; Human; Recombinant (His Tag); Human CD146/MCAM Protein (His Tag); CD146 Protein; MUC18 Protein; melanoma cell adhesion molecule; MCAM recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly559

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of human CD146 precursor (NP_006491.2)(Met 1-Gly 559) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MCAM recombinant protein

Background: The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region, and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumors, and thus has been suggested to play an important role in tumor progression and the development of metastasis in certain human carcinomas.

References

Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.

NCBI and Uniprot Product Information

• NCBI GI #: 71274107
• NCBI GeneID: 4162
• NCBI Accession #: NP_006491.2
• NCBI GenBank Nucleotide #: NM_006500.2
• UniProt Accession #: P43121; O95812; Q59E86; Q6PHR3; Q6ZTR2
• Molecular Weight: 57,605 Da
• NCBI Official Full Name: cell surface glycoprotein MUC18
• NCBI Official Synonym Full Names: melanoma cell adhesion molecule
• NCBI Official Symbol: MCAM
• NCBI Official Synonym Symbols: CD146; MUC18
• NCBI Protein Information: cell surface glycoprotein MUC18
• UniProt Protein Name: Cell surface glycoprotein MUC18
• Protein Family: Cell surface glycoprotein
• UniProt Gene Name: MCAM
• UniProt Synonym Gene Names: MUC18
• UniProt Entry Name: MUC18_HUMAN

Uniprot Description

MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane

Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration