UBE2L6 recombinant protein

Ube2L6 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121206

• Gene Names: UBE2L6; RIG-B; UBCH8
• Purity: >96% as determined by SDS-PAGE
• Synonyms: Ube2L6; Ube2L6 Protein; Human; Recombinant (His Tag); Human UBCH8/UBE2L6 Protein (His Tag); RIG-B Protein; UBCH8 Protein; ubiquitin-conjugating enzyme E2L 6; UBE2L6 recombinant protein

• Host: E Coli
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 0.1% Brij35, pH8.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser153

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human UBE2L6 isoform 1 (O14933-1)(Met 1-Ser 153) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for UBE2L6 recombinant protein

Background: UBCH8, also known as UBE2L6, belongs to the ubiquitin-conjugating enzyme family. The family of ubiquitin-conjugating (E2) enzymes is characterized by the presence of a highly conserved ubiquitin-conjugating (UBC) domain. These domains accommodate the ATP-activated ubiquitin (Ub) or ubiquitin-like (UBL) protein via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the E1 and E3 enzymes and connect activation to covalent modification. By doing so, E2s differentiate effects on downstream substrates, either with a single Ub/UBL molecule or as a chain. UBCH8 is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. It catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. UBCH8 functions in the E6/E6-AP-induced ubiquitination of p53/TP53 and promotes ubiquitination and subsequent proteasomal degradation of FLT3. At protein level, it is present in natural killer cells.

References

Moynihan TP, et al. (1999) The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem. 274(43):30963-8.Ardley HC, et al. (2000) Genomic organization of the human ubiquitin-conjugating enzyme gene, UBE2L6 on chromosome 11q12. Cytogenet Cell Genet. 89(1-2):137-40.Zhao C, et al. (2004) The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-alpha/beta-induced ubiquitin-like protein. Proc Natl Acad. 101(20):7578-82.Tripathi MK, et al. (2005) Down-regulation of UCRP and UBE2L6 in BRCA2 knocked-down human breast cells. Biochem Biophys Res Commun. 328(1):43-8.

NCBI and Uniprot Product Information

• NCBI GI #: 239938941
• NCBI GeneID: 9246
• UniProt Accession #: O14933; Q8N5D8; Q9UEZ0; A6NDM6; A8MY53
• Molecular Weight: 153
• NCBI Official Full Name: Ubiquitin/ISG15-conjugating enzyme E2 L6
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2L 6
• NCBI Official Symbol: UBE2L6
• NCBI Official Synonym Symbols: RIG-B; UBCH8
• NCBI Protein Information: ubiquitin/ISG15-conjugating enzyme E2 L6; ubiquitin-protein ligase L6; ubiquitin carrier protein L6; retinoic acid induced gene B protein; retinoic acid-induced gene B protein
• UniProt Protein Name: Ubiquitin/ISG15-conjugating enzyme E2 L6
• Protein Family: Ubiquitin/ISG15-conjugating enzyme
• UniProt Gene Name: UBE2L6
• UniProt Synonym Gene Names: UBCH8; RIG-B
• UniProt Entry Name: UB2L6_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

UBE2L6: Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: cytosol

Molecular Function: protein binding; ISG15 conjugating enzyme activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: modification-dependent protein catabolic process; ISG15-protein conjugation; cytokine and chemokine mediated signaling pathway; protein modification process; protein ubiquitination; innate immune response; negative regulation of interferon type I production