Mouse Tyrosine, Phosphorylated (HRP) Monoclonal Antibody
Anti-Tyrosine, Phosphorylated (HRP)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
Tyrosine; Phosphorylated (HRP); Monoclonal Antibody; Anti-Tyrosine; anti-Tyrosine; Phosphorylated (HRP) antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
11C162
Specificity
Recognizes phosphorylated tyrosine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not react with nonphosphorylated tyrosine, phosphorylated serine or threonine, AMP, or ATP.
Purity/Purification
Affinity Purified
Form/Format
Supplied as a liquid in stabilizing buffer, 0.1% prolcin-300. Labeled with horseradish peroxidase (HRP). E/P ratio = 4.0.
Applicable Applications for anti-Tyrosine, Phosphorylated (HRP) antibody
ELISA; Western Blot; Immunohistochemistry
Application Notes
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Recommended Dilutions: ELISA: 1:2000-1:10,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:200-1:1000
Recommended Dilutions: ELISA: 1:2000-1:10,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:200-1:1000
Immunogen
O-Phospho-L-tyrosine (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for anti-Tyrosine, Phosphorylated (HRP) antibody
Protein phosphorylation and dephosphorylation reactions is a key postranslational event in the modification of protein functions. Protein phosphorylation occurs at tyrosine, serine, or threonine (p-tyr, p-ser, or p-thr) residues. Many different mitogenic systems, such as the EGF, PDGF, and insulin receptor systems contain tyr/ser/thr kinase domains which autophosphorylate specific tyr, ser or thr residues upon binding of their ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated proteins. An understanding of transformation by oncogenes and mitogenic processes of growth factors requires a delineation of all potential partners involved in phosphorylation cascade. The availability of antibodies specific for p-tyr, p-thr or p-thr residues has greatly advanced the studies on role of phosphorylated proteins.
Product Categories/Family for anti-Tyrosine, Phosphorylated (HRP) antibody
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Product Notes
The Tyrosine, Phosphorylated (HRP) (Catalog #AAA633503) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tyrosine, Phosphorylated (HRP) can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot; Immunohistochemistry. Suitable for use in ELISA, Immunohistochemistry and Western Blot. Recommended Dilutions: ELISA: 1:2000-1:10,000 Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:200-1:1000. Researchers should empirically determine the suitability of the Tyrosine, Phosphorylated (HRP) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tyrosine, Phosphorylated (HRP), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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