Mouse anti-Human TUBA1B Monoclonal Antibody | anti-TUBA1B antibody

TUBA1B (Tubulin alpha-1B Chain, Tubulin alpha-ubiquitous Chain, Alpha-tubulin Ubiquitous, Tubulin K-alpha-1)

Cat. Num. AAA6007467

• Gene Names: TUBA1B; K-ALPHA-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TUBA1B; Monoclonal Antibody; TUBA1B (Tubulin alpha-1B Chain; Tubulin alpha-ubiquitous Chain; Alpha-tubulin Ubiquitous; Tubulin K-alpha-1); Anti -TUBA1B (Tubulin alpha-1B Chain; anti-TUBA1B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 4D1
• Specificity: Recognizes human K-ALPHA-1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MRECISIHVGQAGVQIGNACWELYCLKHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY

• Applicable Applications for anti-TUBA1B antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry.; Dilution: Immunofluorescence: 10ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
• Immunogen: Full length recombinant corresponding to aa1-451 from K-ALPHA-1 (AAH08659) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (75.72kD).)

Western Blot (WB)

(K-ALPHA-1 monoclonal antibody Western Blot analysis of K-ALPHA-1 expression in A-431.)

Western Blot (WB)

(Western Blot analysis of TUBA1B expression in transfected 293T cell line by K-ALPHA-1 monoclonal antibody |Lane 1: TUBA1B transfected lysate (50.2kD).|Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to K-ALPHA-1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to K-ALPHA-1 on A-431 cell. [antibody concentration 10ug/ml])

Related Product Information for anti-TUBA1B antibody

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Product Categories/Family for anti-TUBA1B antibody

Antibodies; Abs to Tubulin

NCBI and Uniprot Product Information

• NCBI GI #: 18204869
• NCBI GeneID: 10376
• UniProt Accession #: P68363; P04687; P05209; Q27I68
• Molecular Weight: 50,152 Da
• NCBI Official Full Name: TUBA1B protein
• NCBI Official Synonym Full Names: tubulin, alpha 1b
• NCBI Official Symbol: TUBA1B
• NCBI Official Synonym Symbols: K-ALPHA-1
• NCBI Protein Information: tubulin alpha-1B chain; alpha tubulin; tubulin K-alpha-1; alpha-tubulin ubiquitous; tubulin, alpha, ubiquitous; tubulin alpha-ubiquitous chain
• UniProt Protein Name: Tubulin alpha-1B chain
• UniProt Gene Name: TUBA1B
• UniProt Entry Name: TBA1B_HUMAN

Uniprot Description

TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: microtubule; cytoplasmic microtubule

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; double-stranded RNA binding; structural molecule activity

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; cytoskeleton-dependent intracellular transport; microtubule cytoskeleton organization and biogenesis; microtubule-based process