Aspartate Aminotransferase Recombinant Protein | AST recombinant protein

Recombinant Aspartate Aminotransferase (AST)

Cat. Num. AAA2030599

• Gene Names: ASP5; AAT3; ASP5; ASPARTATE AMINOTRANSFERASE; aspartate aminotransferase 5; ASPARTATE AMINOTRANSFERASE DEFICIENT 3; ATAAT1; F11C18.15
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Aspartate Aminotransferase; Recombinant Aspartate Aminotransferase (AST); AST recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; APPSIFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDSQPWV LPVVRKVEQRIANDSSINHE YLPILGLAEF RTCASRLALG DDSPALQEKR VGGVQCLGGT GALRIGAEFLARWYNGTNNK DTPVYVSSPT WENHNGVFIA AGFKDIRSYH YWDAAKRGLD LQGFLNDLEKAPEFSIFVLH ACAHNPTGTD PTPEQWKQIA SVMKRRFLFP FFDSAYQGFA SGSLEKDAWAIRYFVSEGFE LFCAQSFSKN FGLYNERVGN LTVVAKEPDS ILRVLSQMEK IVRITWSNPPAQGARIVART LSDPELFNEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITEQIGMFSFTGLNPKQ VEYLINEKHI YLLPSGRINM CGLTTKNLEY VATSIHEAVT KIQ
• Sequence Length: 448

• Applicable Applications for AST recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 50.0KDa
• Accurate Molecular Mass (KD): 50kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Bos taurus; Bovine (Cattle)
• Fragment: Ala2~Gln413
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 334187077
• NCBI GeneID: 829330
• NCBI Accession #: NP_001190885.1
• NCBI GenBank Nucleotide #: NM_001203956.1
• Molecular Weight: 49,831 Da
• NCBI Official Full Name: aspartate aminotransferase
• NCBI Official Symbol: ASP5
• NCBI Official Synonym Symbols: AAT3; ASP5; ASPARTATE AMINOTRANSFERASE; aspartate aminotransferase 5; ASPARTATE AMINOTRANSFERASE DEFICIENT 3; ATAAT1; F11C18.15
• NCBI Protein Information: aspartate aminotransferase
• UniProt Protein Name: Aspartate aminotransferase, chloroplastic
• Protein Family: Allatostatin
• UniProt Gene Name: ASP5
• UniProt Entry Name: AAT5_ARATH

NCBI Description

encodes a plastid-localized aspartate aminotransferase

Uniprot Description

Amino acid aminotransferase important for the metabolism of amino acids and Krebs-cycle related organic acids. No activity with D-Asp or D-Ala as amino donors. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.