Rat Granulocyte Colony Stimulating Factor ELISA Kit | G-CSF elisa kit

Rat Granulocyte Colony Stimulating Factor ELISA Kit

Cat. Num. AAA728668

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33; MGC45931
• Reactivity: Rat
• Synonyms: Granulocyte Colony Stimulating Factor; Rat Granulocyte Colony Stimulating Factor ELISA Kit; Rat Granulocyte Colony Stimulating Factor (G-CSF) ELISA Kit; Granulocyte Colony Stimulating Factor (Rat); G-CSF elisa kit

• Reactivity: Rat

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for G-CSF elisa kit

Intended Uses: This G-CSF ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of G-CSF in the sample, this G-CSF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus G-CSF concentration. The concentration of G-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This G-CSF enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for G-CSF. Standards or samples are then added to the microtiter plate wells and G-CSF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of G-CSF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for G-CSF are added to each well to "sandwich" the G-CSF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain G-CSF and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for G-CSF elisa kit

Rat ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 297660573
• NCBI GeneID: 1440
• UniProt Accession #: P09919; Q5FBY7; Q6FH65; Q8N4W3
• Molecular Weight: 22,293 Da
• NCBI Official Full Name: granulocyte colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33; MGC45931
• NCBI Protein Information: granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin; OTTHUMP00000164325; OTTHUMP00000164326; OTTHUMP00000164327; OTTHUMP00000164328
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF
• UniProt Entry Name: CSF3_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]

Uniprot Description

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Post-translational modification: O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

Pharmaceutical use: Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).

Sequence similarities: Belongs to the IL-6 superfamily.

Caution: Ref.5 misquotes the gene name as "CSF1".