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Typical Testing Data/Standard Curve (for reference only)

Human lectin, galactoside-binding, soluble, 9 ELISA Kit | LGALS9 elisa kit

Human Galectin-9, LGALS9 ELISA Kit

Gene Names
LGALS9; HUAT; LGALS9A
Reactivity
Human
Synonyms
lectin; galactoside-binding; soluble; 9; Human Galectin-9; LGALS9 ELISA Kit; Human Galectin-9 (LGALS9) ELISA kit; HUAT; LGALS9A; MGC117375; MGC125973; MGC125974; ecalectin; galectin 9; galectin-9; urate transporter/channel protein; LGALS9 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human LGALS9. No significant cross-reactivity or interference between Human LGALS9 and analogues was observed.
Sequence Length
323
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LGALS9 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LGALS9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LGALS9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,518 Da
NCBI Official Full Name
galectin-9 isoform short
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 9
NCBI Official Symbol
LGALS9
NCBI Official Synonym Symbols
HUAT; LGALS9A
NCBI Protein Information
galectin-9; gal-9; ecalectin; galectin 9; tumor antigen HOM-HD-21; urate transporter/channel protein
UniProt Protein Name
Galectin-9
Protein Family
UniProt Gene Name
LGALS9
UniProt Synonym Gene Names
Gal-9
UniProt Entry Name
LEG9_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm

Molecular Function: signal transducer activity; galactose binding; carbohydrate binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction

Research Articles on LGALS9

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Product Notes

The Human LGALS9 lgals9 (Catalog #AAA905376) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA905376 ELISA Kit recognizes Human LGALS9. It is sometimes possible for the material contained within the vial of "lectin, galactoside-binding, soluble, 9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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