Mouse anti-Human TrCP, beta Monoclonal Antibody | anti-Rela antibody

TrCP, beta

Cat. Num. AAA600531

• Gene Names: Rela; p65
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TrCP; beta; Monoclonal Antibody; Anti -TrCP; anti-Rela antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6A200
• Specificity: Recognizes human beta-TrCP at ~70kD. No crossreactivity with other proteins in Western Blot applications. Reactivity has been confirmed with human beta-TrCP-transfected HEK293 cell lysates.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-Rela antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and ELISA.; Dilution: Western Blot: 1-3ug/ml; Immunoprecipitation: 7ug/IP reaction; ELISA: 0.1-1ug/ml
• Immunogen: Recombinant human beta-TrCP fusion protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HEK 293 cells transfected with b-TrCP using MBS600531.)

Related Product Information for anti-Rela antibody

Beta-TrCP plays a relevant role in the control of stability of several key protein factors. In Xenopus, beta-TrCP acts as an inhibitor of Wnt signaling and dorsal axis formation. A variety of cellular stress can induce the level of beta-TrCP mRNA and protein levels in human cells. Activation of stress-activated protein kinases JNK by forced expression of constitutively active

Product Categories/Family for anti-Rela antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 417926
• NCBI GeneID: 19697
• UniProt Accession #: Q04207; Q62025
• Molecular Weight: 60,212 Da
• NCBI Official Full Name: Transcription factor p65
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• NCBI Official Symbol: Rela
• NCBI Official Synonym Symbols: p65
• NCBI Protein Information: transcription factor p65; p65 NFkB; p65 NF kappaB; p65 NF-kappa B; nuclear factor NF-kappa-B p65 subunit; avian reticuloendotheliosis viral (v-rel) oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
• UniProt Protein Name: Transcription factor p65
• Protein Family: Transcription factor
• UniProt Gene Name: Rela
• UniProt Synonym Gene Names: Nfkb3
• UniProt Entry Name: TF65_MOUSE

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: DNA-binding; Transcription factor; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; transcription activator binding; protein N-terminus binding; protein kinase binding; transcription factor binding; NF-kappaB binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; chromatin binding; phosphate binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; cellular response to stress; negative regulation of insulin receptor signaling pathway; defense response; negative regulation of transcription from RNA polymerase II promoter; activation of NF-kappaB transcription factor; response to organic substance; hair follicle development; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; inflammatory response; positive regulation of Schwann cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; liver development; response to UV-B; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; positive regulation of interleukin-12 biosynthetic process; positive regulation of chondrocyte differentiation; response to bacterium; response to muramyl dipeptide; response to cytokine stimulus; regulation of inflammatory response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process; negative regulation of apoptosis