Mouse DCP2 (Decapping mRNA 2) Monoclonal Antibody | anti-DCP2 antibody

DCP2 (Decapping mRNA 2) Mouse Monoclonal Antibody [Clone PCRP-DCP2-1D6]

Cat. Num. AAA4381827

• Gene Names: DCP2; NUDT20
• Reactivity: Human; Predicted to react with Mouse and Rat.
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: DCP2 (Decapping mRNA 2); Monoclonal Antibody; DCP2 (Decapping mRNA 2) Mouse Monoclonal Antibody [Clone PCRP-DCP2-1D6]; DCP2 decapping enzyme homolog (S. cerevisiae); DFLJ33245; hDpc; m7GpppN-mRNA hydrolase; mRNA decapping enzyme 2; nudix (nucleoside diphosphate linked moiety X)-type motif 20 (NUDT20); anti-DCP2 antibody

• Host: Mouse
• Reactivity: Human; Predicted to react with Mouse and Rat.
• Clonality: Monoclonal
• Isotype: Mouse/IgG2a
• Clone Number: PCRP-DCP2-1D6
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-DCP2 antibody: Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: IP: 1-2ug per 100-500ug of total protein); FC/FACS: 1-2ug/million cells; IF: 1-2ug/ml
• Immunogen: Recombinant full-length human DCP2 protein
• Cellular Localization: Cytoplasm > P, body, Nucleus, Predominantly cytoplasmic, in processing bodies (PB), A minor amount is nuclear
• Positive Control: MCF7, Daudi or Jurkat cells.
• Hu-chromosome Location: 5q22.2
• Unigene: 443875
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified DCP2 Mouse Monoclonal Antibody (PCRP-DCP2-1D6). Confirmation of Purity and Integrity of Antibody. )

Related Product Information for anti-DCP2 antibody

The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3' exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay

NCBI and Uniprot Product Information

• NCBI GI #: 31542498
• NCBI GeneID: 167227
• NCBI Accession #: NP_689837
• NCBI GenBank Nucleotide #: NM_152624.5
• UniProt Accession #: Q8IU60; Q6P2D4; Q7Z5W5; Q8NBG5; C9J778
• Molecular Weight: 46 kDa
• NCBI Official Full Name: m7GpppN-mRNA hydrolase isoform 1
• NCBI Official Synonym Full Names: decapping mRNA 2
• NCBI Official Symbol: DCP2
• NCBI Official Synonym Symbols: NUDT20
• NCBI Protein Information: m7GpppN-mRNA hydrolase; hDpc; mRNA-decapping enzyme 2; DCP2 decapping enzyme homolog; nudix (nucleoside diphosphate linked moiety X)-type motif 20
• UniProt Protein Name: m7GpppN-mRNA hydrolase
• Protein Family: mRNA-decapping enzyme
• UniProt Gene Name: DCP2
• UniProt Synonym Gene Names: NUDT20; Nudix motif 20; hDpc
• UniProt Entry Name: DCP2_HUMAN

NCBI Description

The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

DCP2: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7- methyl guanine cap structure from mRNA molecules, yielding a 5'- phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety. Belongs to the Nudix hydrolase family. DCP2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; RNA processing; EC 3.6.1.62

Chromosomal Location of Human Ortholog: 5q22.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cell junction; cytosol

Molecular Function: protein binding; m7G(5')pppN diphosphatase activity; exoribonuclease activity, producing 5'-phosphomonoesters; RNA binding; manganese ion binding

Biological Process: mRNA catabolic process; mRNA catabolic process, nonsense-mediated decay; gene expression; mRNA catabolic process, deadenylation-dependent decay