anti-Mouse, Rat NF-kB p65 Polyclonal Antibody | anti-NF-kB p65 antibody

Anti-NF-kB p65 Antibody

Cat. Num. AAA178306

• Gene Names: Rela; p65
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: NF-kB p65; Polyclonal Antibody; Anti-NF-kB p65 Antibody; Transcription factor p65; Avian reticuloendotheliosis viral (v rel) oncogene homolog A; MGC131774; NF kappa B p65delta3; NFKB 3; NFKB3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 p65 (avian); OTTHUMP00000233473; OTTHUMP00000233474; OTTHUMP00000233475; OTTHUMP00000233476; OTTHUMP00000233900; p65; p65 NF kappaB; p65 NFkB; RELA; TF65_HUMAN antibodyTranscription Factor p65; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)); V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; v rel reticuloendotheliosis viral oncogene homolog A (avian); v rel reticuloendotheliosis viral oncogene homolog A; V rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B cells 3; V-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel avian reticuloendotheliosis viral oncogene homolog A; anti-NF-kB p65 antibody

• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 549

• Applicable Applications for anti-NF-kB p65 antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: E Coli-derived mouse NF-kB p65 recombinant protein (Position: D291-Q479). Mouse NF-kB p65 shares 77% amino acid (aa) sequence identity with human NF-kB p65.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- NF-kB p65 Picoband antibody, MBS178306, Western blottingAll lanes: Anti NF-kB p65 (MBS178306) at 0.5ug/mlgLane 1: PC 12 Whole Cell Lysate at 40ugLane 2: NRK Whole Cell Lysate at 40ugLane 3: HEPA Whole Cell Lysate at 40ugLane 4: NIH Whole Cell Lysate at 40ugPredicted bind size: 65KDObserved bind size: 65KD )

Related Product Information for anti-NF-kB p65 antibody

Description: Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB in Mouse;Rat.

Background: Transcription factor p65, also known as NFKB3 or NF-kB p65, is a protein encoded by the RELA gene. NFKB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc, and it may play a role in inflammatory conditions of the peripheral nervous system. Phosphorylation and acetylation of NFKB3 are crucial post-translational modifications required for NFKB activation. It has also been shown to modulate immune responses, and activation of NFKB3 is positively associated with multiple types of cancer. In addition to that, NFKB3 antagonizes TNFR1-JNK proliferative signals in epidermis and plays a nonredundant role in restraining epidermal growth.

References

1. Nolan GP, Ghosh S, Liou HC, Tempst P, Baltimore D (April 1991). "DNA binding and I kappa B inhibition of the cloned p65 subunit of NF-kappa B, a rel-related polypeptide". Cell 64 (5): 961-9. 2. Lim, C.-A., Yao, F., Wong, J. J.-Y., George, J., Xu, H., Chiu, K. P., Sung, W.-K., Lipovich, L., Vega, V. B., Chen, J., Shahab, A., Zhao, X. D., Hibberd, M., Wei, C.-L., Lim, B., Ng, H.-H., Ruan, Y., Chin, K.-C. Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappa-B upon TLR4 activation.Molec. Cell 27: 622-635, 2007. 3. Mazzeo, A., Aguennouz, M., Messina, C., Vita, G. Immunolocalization and activation of transcription factor nuclear factor kappa-B in dysimmune neuropathies and familial amyloidotic polyneuropathy. Arch. Neurol. 61: 1097-1102, 2004.

NCBI and Uniprot Product Information

• NCBI GI #: 6677709
• NCBI GeneID: 19697
• NCBI Accession #: NP_033071.1
• NCBI GenBank Nucleotide #: NM_009045.4
• UniProt Accession #: Q04207; Q62025
• Molecular Weight: 59,061 Da
• NCBI Official Full Name: transcription factor p65
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• NCBI Official Symbol: Rela
• NCBI Official Synonym Symbols: p65
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: Rela
• UniProt Synonym Gene Names: Nfkb3
• UniProt Entry Name: TF65_MOUSE

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor

Cellular Component: cytoplasm; cytosol; nucleus; protein complex; transcription factor complex

Molecular Function: actinin binding; chromatin binding; chromatin DNA binding; DNA binding; enzyme binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein complex binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; cellular response to stress; cytokine and chemokine mediated signaling pathway; defense response; hair follicle development; inflammatory response; innate immune response; liver development; negative regulation of apoptosis; negative regulation of insulin receptor signaling pathway; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; organ morphogenesis; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 biosynthetic process; positive regulation of Schwann cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; regulation of transcription, DNA-dependent; response to bacterium; response to cytokine stimulus; response to muramyl dipeptide; response to organic substance; response to UV-B; transcription from RNA polymerase II promoter; transcription, DNA-dependent