Testing Data
(25 ug murine EL4 whole cell lysate (lanes 2, and 3) was loaded onto a 4-15% Criterion„¢ TGX-Stain-Free„¢ Gel and transferred onto a nitrocellulose membrane. The membrane was blocked with Block ACE (BUF029) and probed with rat anti TOLLIP antibody at 5ug/ml in Block ACE wash (Lane 2) for 1 hour at room temperature. The membrane was washed 3x for 10 minutes with 5 ml Block ACE wash before addition of the HRP-conjugated goat anti-Rat IgG secondary antibody at 1/10,000 (Lanes 1-3). The membrane was washed 4x for 5 minutes with Block ACE wash and 2x in 5 ml PBS before developing with Clarity ECL on the Chemidoc. Lane 3 is a secondary only antibody control)
Rat anti-Mouse, Human TOLLIP Monoclonal Antibody | anti-Tollip antibody
Rat anti TOLLIP
Gene Names
Tollip; 4930403G24Rik; 4931428G15Rik
Reactivity
Mouse, Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
TOLLIP; Monoclonal Antibody; Rat anti TOLLIP; TOLLIP Antibody; anti-Tollip antibody
Host
Rat
Reactivity
Mouse, Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
Kimmy-1
Purity/Purification
Purified
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide (NaN3).
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
274
Applicable Applications for anti-Tollip antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
WB: 5 ug/ml
Immunogen
Recombinant mouse TOLLIP
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Testing Data
(25 ug murine EL4 whole cell lysate (lanes 2, and 3) was loaded onto a 4-15% Criterion„¢ TGX-Stain-Free„¢ Gel and transferred onto a nitrocellulose membrane. The membrane was blocked with Block ACE (BUF029) and probed with rat anti TOLLIP antibody at 5ug/ml in Block ACE wash (Lane 2) for 1 hour at room temperature. The membrane was washed 3x for 10 minutes with 5 ml Block ACE wash before addition of the HRP-conjugated goat anti-Rat IgG secondary antibody at 1/10,000 (Lanes 1-3). The membrane was washed 4x for 5 minutes with Block ACE wash and 2x in 5 ml PBS before developing with Clarity ECL on the Chemidoc. Lane 3 is a secondary only antibody control)
Testing Data
(25 ug murine EL4 whole cell lysate (lanes 2, and 3) was loaded onto a 4-15% Criterion„¢ TGX-Stain-Free„¢ Gel and transferred onto a nitrocellulose membrane. The membrane was blocked with Block ACE (BUF029) and probed with rat anti TOLLIP antibody at 5ug/ml in Block ACE wash (Lane 2) for 1 hour at room temperature. The membrane was washed 3x for 10 minutes with 5 ml Block ACE wash before addition of the HRP-conjugated goat anti-Rat IgG secondary antibody at 1/10,000 (Lanes 1-3). The membrane was washed 4x for 5 minutes with Block ACE wash and 2x in 5 ml PBS before developing with Clarity ECL on the Chemidoc. Lane 3 is a secondary only antibody control)
Related Product Information for anti-Tollip antibody
Rat anti TOLLIP antibody clone Kimmy-1 recognizes Toll-interacting protein (TOLLIP), an ubiquitin binding protein that plays an important role in regulating the innate immune response.
TOLLIP, as the name implies, interacts with a number of Toll-like receptors thereby mediating the regulation of the autophagic clearance and NF-kappaB pathways. The latter pathway, TOLLIP mainly regulates through controlling phosphorylation levels of the IRAK1 kinase. TOLLIP deficiencies have been implicated in increased tuberculosis susceptibility (Sha et al. 2012).
TOLLIP, as the name implies, interacts with a number of Toll-like receptors thereby mediating the regulation of the autophagic clearance and NF-kappaB pathways. The latter pathway, TOLLIP mainly regulates through controlling phosphorylation levels of the IRAK1 kinase. TOLLIP deficiencies have been implicated in increased tuberculosis susceptibility (Sha et al. 2012).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,345 Da
NCBI Official Full Name
Toll-interacting protein
NCBI Official Synonym Full Names
toll interacting protein
NCBI Official Symbol
Tollip
NCBI Official Synonym Symbols
4930403G24Rik; 4931428G15Rik
NCBI Protein Information
toll-interacting protein
UniProt Protein Name
Toll-interacting protein
Protein Family
UniProt Gene Name
Tollip
Uniprot Description
Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates ().
Research Articles on Tollip
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Product Notes
The Tollip tollip (Catalog #AAA225373) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat anti TOLLIP reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's TOLLIP can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). WB: 5 ug/ml. Researchers should empirically determine the suitability of the Tollip tollip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOLLIP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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