Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Toll-interacting protein (TOLLIP) Standard Curve.)
Mouse Toll-Interacting Protein (TOLLIP) ELISA Kit | TOLLIP elisa kit
Mouse Toll-Interacting Protein (TOLLIP) ELISA Kit
Gene Names
Tollip; 4930403G24Rik; 4931428G15Rik
Reactivity
Mouse
Synonyms
Toll-Interacting Protein (TOLLIP); Mouse Toll-Interacting Protein (TOLLIP) ELISA Kit; TOLLIP; FLJ33531; IL-1RAcPIP; Toll-interacting protein; adapter protein; TOLLIP elisa kit
Reactivity
Mouse
Specificity
Mouse Toll-interacting protein (TOLLIP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TOLLIP. No significant cross-reactivity or interference between Mouse TOLLIP and analogues was observed.
Sequence Length
274
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Toll-interacting protein (TOLLIP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Toll-interacting protein (TOLLIP) Standard Curve.)
Related Product Information for TOLLIP elisa kit
Description: This Mouse Toll-interacting protein (TOLLIP) ELISA Kit employs a two-site sandwich ELISA to quantitate TOLLIP in samples. An antibody specific for TOLLIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOLLIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOLLIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOLLIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Toll interacting protein, also known as TOLLIP, is an inhibitory adaptor protein.It is an inhibitory adaptor protein within Toll-like receptors (TLR). The TLR pathway is a part of the innate immune system that recognizes structurally conserved molecular patterns of microbial pathogens, leading to an inflammatory immune response. Compared with the full-length isoform, 2 of the human proteins lack the N-terminal TBD, and 1 lacks the C2 domain. A mouse-specific isoform lacks the C-terminal CUE domain. EST database analysis suggested that full-length human TOLLIP is ubiquitously expressed. Expression of the other human variants appeared to be more restricted, with evidence for enrichment in brain.
Background: Toll interacting protein, also known as TOLLIP, is an inhibitory adaptor protein.It is an inhibitory adaptor protein within Toll-like receptors (TLR). The TLR pathway is a part of the innate immune system that recognizes structurally conserved molecular patterns of microbial pathogens, leading to an inflammatory immune response. Compared with the full-length isoform, 2 of the human proteins lack the N-terminal TBD, and 1 lacks the C2 domain. A mouse-specific isoform lacks the C-terminal CUE domain. EST database analysis suggested that full-length human TOLLIP is ubiquitously expressed. Expression of the other human variants appeared to be more restricted, with evidence for enrichment in brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Toll-interacting protein
NCBI Official Synonym Full Names
toll interacting protein
NCBI Official Symbol
Tollip
NCBI Official Synonym Symbols
4930403G24Rik; 4931428G15Rik
NCBI Protein Information
toll-interacting protein
UniProt Protein Name
Toll-interacting protein
Protein Family
UniProt Gene Name
Tollip
Uniprot Description
Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates ().
Research Articles on TOLLIP
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Product Notes
The Mouse TOLLIP tollip (Catalog #AAA9716691) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716691 ELISA Kit recognizes Mouse TOLLIP. It is sometimes possible for the material contained within the vial of "Toll-Interacting Protein (TOLLIP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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