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Typical Testing Data/Standard Curve (for reference only)

Rat Tyrosine Hydroxylase (TH) DIY ELISA Kit | TH diy elisa kit

Rat Tyrosine Hydroxylase (TH) ELISA Kit DIY Materials

Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Rat
Synonyms
Tyrosine Hydroxylase (TH); Rat Tyrosine Hydroxylase (TH) ELISA Kit DIY Materials; TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14; TH diy elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Tyrosine Hydroxylase (TH). No significant cross-reactivity or interference between Tyrosine Hydroxylase (TH) and analogues was observed.
Sequence Length
106
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.122ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for TH diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
44,898 Da
NCBI Official Full Name
tyrosine hydroxylase, partial
NCBI Official Synonym Full Names
tyrosine hydroxylase
NCBI Official Symbol
TH
NCBI Official Synonym Symbols
TYH; DYT14; DYT5b
NCBI Protein Information
tyrosine 3-monooxygenase
UniProt Protein Name
Tyrosine 3-monooxygenase
UniProt Gene Name
TH
UniProt Synonym Gene Names
TYH; TH

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Amino Acid Metabolism - tyrosine; EC 1.14.16.2; Endoplasmic reticulum; Mitochondrial; Oxidoreductase; Vesicle

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; internal side of plasma membrane; melanosome membrane; neuron projection; nucleus; perikaryon; smooth endoplasmic reticulum

Molecular Function: enzyme binding; protein binding; tyrosine 3-monooxygenase activity

Biological Process: anatomical structure morphogenesis; catecholamine biosynthetic process; dopamine biosynthetic process; embryonic camera-type eye morphogenesis; epinephrine biosynthetic process; eye photoreceptor cell development; heart development; learning; locomotory behavior; memory; norepinephrine biosynthetic process; organ morphogenesis; pigmentation; regulation of heart contraction; response to ethanol; response to hypoxia; synaptic transmission, dopaminergic; visual perception

Disease: Segawa Syndrome, Autosomal Recessive

Research Articles on TH

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Product Notes

The Rat TH th (Catalog #AAA2089097) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089097 DIY ELISA Kit recognizes Rat TH. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the TH th for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tyrosine Hydroxylase (TH), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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