Rabbit MAP3K10 Polyclonal Antibody | anti-MAP3K10 antibody

MAP3K10 Polyclonal Antibody

Cat. Num. AAA3007191

• Gene Names: MAP3K10; MST; MLK2; MEKK10
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MAP3K10; Polyclonal Antibody; MAP3K10 Polyclonal Antibody; Mitogen-activated protein kinase kinase kinase 10 (EC: 2.7.11.25); Mixed lineage kinase 2; Protein kinase MST; MLK2; MST; anti-MAP3K10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MAP3K10 polyclonal antibody detects endogenous levels of MAP3K10 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 954

• Applicable Applications for anti-MAP3K10 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MAP3K10.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MAP3K10 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:RAW264. 7 whole cell lysate Lane3:H9C2 whole cell lysateLane4:HELA whole cell lysate)

Related Product Information for anti-MAP3K10 antibody

Mixed lineage kinases are a family of protein kinases sharing two leucine zipper-like motifs, which are known to mediate protein dimerization, and a kinasen domain whose primary structure is similar to both the tyrosine-specific and the serine/threonine-specific kinase classes. Members of the mixed-lineage kinase (MLK) family include MLK1, MLK2, MLK3 and dual leucine zipper kinase, also designated DLK. MLKs are expressed in neuronal cells where they are likely to interact between Rac1/Cdc42, MKK4 and MKK7 in death signaling. The human MLK1 gene maps to chromosome 14q24.3-q31 and is expressed in epithelial tumor cell lines of the colon, breast, and esophagus. The human MLK2 gene maps to chromosome 19 q13.2. and encodes a predicted 954 amino acid, src homology 3 (SH3) domain-containing protein. The human MLK3 gene maps to chromosome 11q13.1-13.3 and encodes a 847 amino acid, SH3 domain- and proline rich region-containing protein. Apoptosis mechanisms rely on MLKs as an upstream intermediate of mitochondrial cytochrome c release and caspase activation.

NCBI and Uniprot Product Information

• NCBI GI #: 21735550
• NCBI GeneID: 4294
• NCBI Accession #: NP_002437.2
• NCBI GenBank Nucleotide #: NP_002437.2
• UniProt Accession #: Q02779
• Molecular Weight: ~ 104kDa
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 10
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 10
• NCBI Official Symbol: MAP3K10
• NCBI Official Synonym Symbols: MST; MLK2; MEKK10
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 10
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 10
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K10
• UniProt Synonym Gene Names: MLK2; MST
• UniProt Entry Name: M3K10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

MLK2: a TKL kinase of the MLK family. A dual leucine zipper kinase. Phosphorylates and activates JNK and SEK1. Interacts with PAK and NCK. May be involved in nerve growth factor-induced neuronal apoptosis.

Protein type: Kinase, protein; EC 2.7.11.25; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; JUN kinase kinase kinase activity; protein homodimerization activity; bHLH transcription factor binding; transcription corepressor activity; ATP binding; protein kinase activity

Biological Process: positive regulation of JNK activity; smoothened signaling pathway; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; negative regulation of transcription factor activity; peptidyl-threonine phosphorylation; positive regulation of JNK cascade; signal transduction; activation of JNK activity; peptidyl-serine phosphorylation; JNK cascade; negative regulation of transcription, DNA-dependent; activation of JNKK activity