TIGIT active protein

TIGIT Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8123041

• Gene Names: Tigit; Vstm3
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TIGIT; TIGIT Protein; Mouse; Recombinant (hFc Tag); Mouse TIGIT/VSTM3 Protein (Fc Tag); ENSMUSG00000071552 Protein; Vstm3 Protein; T cell immunoreceptor with Ig and ITIM domains; TIGIT active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly141

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 26
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse TIGIT (PP86176)(Met1-Gly141) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Immobilized mouse PVR-His at 10ug/ml (100uL/well) can bind mouse TIGIT-Fc. The EC50 of mouse TIGIT-Fc is 0.25-0.55ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Immobilized mouse PVR-His at 10 ug/ml (100 ul/well) can bind mouse TIGIT-Fc, The EC50 of mouse TIGIT-Fc is 0.25-0.55 ug/ml.)

Related Product Information for TIGIT active protein

Background: TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.

Product Categories/Family for TIGIT active protein

ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

References

Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat. Genet. 36:40-45.Bechtel S., et al., (2007), The full-ORF clone resource of the German cDNA consortium.BMC Genomics 8:399-399.Yu X., Harden K., et al.,(2009), The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. Nat. Immunol. 10:48-57.

NCBI and Uniprot Product Information

• NCBI GI #: 226423924
• NCBI GeneID: 100043314
• NCBI Accession #: NP_001139797.1; PP86176
• NCBI GenBank Nucleotide #: NM_001146325.1
• Molecular Weight: 26,959 Da
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: Tigit
• NCBI Official Synonym Symbols: Vstm3
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• Protein Family: T-cell immunoreceptor with Ig and ITIM domains