SDS-Page
(SDS-PAGE Analysis Purified TDRKH Mouse Monoclonal Antibody (PCRP-TDRKH-1H2). Confirmation of Purity and Integrity of Antibody. )
Mouse TDRKH Monoclonal Antibody | anti-TDRKH antibody
TDRKH Mouse Monoclonal Antibody [Clone PCRP-TDRKH-1H2]
Gene Names
TDRKH; TDRD2
Reactivity
HumanPredicted to react with Mouse and Rat.
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
TDRKH; Monoclonal Antibody; TDRKH Mouse Monoclonal Antibody [Clone PCRP-TDRKH-1H2]; 2700091C21Rik; Putative RNA binding protein; RGD1311196; RP11-98D18. 8; TDRD2; TDRD7; Tdrkh; Tudor and KH domain containing; Tudor and KH domain-containing protein; tudor domain containing 2; anti-TDRKH antibody
Host
Mouse
Reactivity
Human
Predicted to react with Mouse and Rat.
Predicted to react with Mouse and Rat.
Clonality
Monoclonal
Isotype
Mouse/IgG2b
Clone Number
PCRP-TDRKH-1H2
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-TDRKH antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Formalin (IHC-F)
Application Notes
IP: 1-2ug per 100-500ug of total protein)
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
WB: 1-2ug/ml
IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
WB: 1-2ug/ml
IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
Immunogen
Recombinant full-length human TDRKH protein
Cellular Localization
Cytoplasm, Localized to cytosol and centrosome
Positive Control
HeLa or MCF7 cells. Human testis or brain.
Hu-chromosome Location
1q21.3
Unigene
144439
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-Page
(SDS-PAGE Analysis Purified TDRKH Mouse Monoclonal Antibody (PCRP-TDRKH-1H2). Confirmation of Purity and Integrity of Antibody. )
SDS-Page
(SDS-PAGE Analysis Purified TDRKH Mouse Monoclonal Antibody (PCRP-TDRKH-1H2). Confirmation of Purity and Integrity of Antibody. )
Related Product Information for anti-TDRKH antibody
Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
561
NCBI Official Full Name
Tudor and KH domain-containing protein
NCBI Official Synonym Full Names
tudor and KH domain containing
NCBI Official Symbol
TDRKH
NCBI Official Synonym Symbols
TDRD2
NCBI Protein Information
tudor and KH domain-containing protein; tudor domain containing 2; putative RNA binding protein; tudor domain-containing protein 2; tudor and KH domain containing protein
UniProt Protein Name
Tudor and KH domain-containing protein
Protein Family
UniProt Gene Name
TDRKH
UniProt Synonym Gene Names
TDRD2
UniProt Entry Name
TDRKH_HUMAN
Uniprot Description
TDRKH: a protein containing one Tudor and two KH_1 domims. Tudor domains have been shown to interact with arginine-glycine-rich motifs in a methylarginine-dependent manner, and target methylated arginines physiologically. KH domains are RNA-binding domains found in a wide variety of nucleic acid-binding proteins. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: mitochondrion
Molecular Function: RNA binding
Biological Process: fertilization; DNA methylation during gametogenesis; RNA-mediated gene silencing; gene expression; spermatogenesis; cell differentiation; male meiosis
Research Articles on TDRKH
Similar Products
Product Notes
The TDRKH tdrkh (Catalog #AAA4381767) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TDRKH Mouse Monoclonal Antibody [Clone PCRP-TDRKH-1H2] reacts with Human Predicted to react with Mouse and Rat. and may cross-react with other species as described in the data sheet. AAA Biotech's TDRKH can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Formalin (IHC-F). IP: 1-2ug per 100-500ug of total protein) FC/FACS: 1-2ug/million cells IF: 1-2ug/ml WB: 1-2ug/ml IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the TDRKH tdrkh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TDRKH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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