Testing Data
(C57BL/6 splenocytes stained with CD3 APC and MBS642143, followed by anti-rat IgG FITC.)
Rat anti-Mouse TCR, V beta 8.1, 8.2 Monoclonal Antibody
TCR, V beta 8.1, 8.2 (T Cell Receptor Vb8.1, 8.2 Chain, T Cell Receptor)
Reactivity
Mouse
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
TCR; V beta 8.1; 8.2; Monoclonal Antibody; 8.2 (T Cell Receptor Vb8.1; 8.2 Chain; T Cell Receptor); Anti -TCR; anti-TCR; 8.2 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Specificity
Recognizes mouse TCR Vb8.1, 8.2.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-TCR, V beta 8.1, 8.2 antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry and Immunoprecipitation.
Dilution: Flow Cytometry: 0.25ug/10e6 cells in 100ul volume
Dilution: Flow Cytometry: 0.25ug/10e6 cells in 100ul volume
Immunogen
TCR receptor from T cell hybridoma DO-11.10
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(C57BL/6 splenocytes stained with CD3 APC and MBS642143, followed by anti-rat IgG FITC.)
Testing Data
(C57BL/6 splenocytes stained with CD3 APC and MBS642143, followed by anti-rat IgG FITC.)
Related Product Information for anti-TCR, V beta 8.1, 8.2 antibody
The TCR Vb 8 family consists of three members, namely Vb8.1, 8.2 and 8.3. This antibody reacts with Vb8.1, 8.2 only, but not Vb8.3. Most mouse strains have about 20% Vb 8+ T cells in the periphery. However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrc haplotype) do not express Vb8 due to a gene deletion at the Vb 8 family loci. Other strains expressing endogenous super-antigen, such as Mls-1a have significant reduced number of Vb 8.1 cells in the periphery. Exogenous superantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vb 8 and selectively eliminate those T cells in vivo.
Product Categories/Family for anti-TCR, V beta 8.1, 8.2 antibody
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Product Notes
The TCR, V beta 8.1, 8.2 (Catalog #AAA642143) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The TCR, V beta 8.1, 8.2 (T Cell Receptor Vb8.1, 8.2 Chain, T Cell Receptor) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TCR, V beta 8.1, 8.2 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry and Immunoprecipitation. Dilution: Flow Cytometry: 0.25ug/10e6 cells in 100ul volume. Researchers should empirically determine the suitability of the TCR, V beta 8.1, 8.2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR, V beta 8.1, 8.2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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