Rabbit anti-Human ABCG1 Polyclonal Antibody | anti-ABCG1 antibody

ABCG1, NT (ATP-binding Cassette Sub-family G Member 1, ATP-binding Cassette Transporter 8, White Protein Homolog, WHT1, ABC8) (HRP)

Cat. Num. AAA6460863

• Gene Names: ABCG1; ABC8; WHITE1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ABCG1; Polyclonal Antibody; NT (ATP-binding Cassette Sub-family G Member 1; ATP-binding Cassette Transporter 8; White Protein Homolog; WHT1; ABC8) (HRP); anti-ABCG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ABCG1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ABCG1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ABCG1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of ABCG1 Antibody (Center) in 293 cell line lysates (35ug/lane). ABCG1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ABCG1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ABCG1 Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ABCG1 antibody

Antibodies; Multi Drug Resistance (MDR)

References

1. Furuyama, S., J. Atheroscler. Thromb. 16(3), 194-200 (2009). 2. Stefulj, J., Circ. Res. 104(5), 600-608 (2009). 3. Mauerer, R., Exp. Mol. Med. 41(2), 126-132 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 46592915
• NCBI GeneID: 9619
• NCBI Accession #: NP_004906
• NCBI GenBank Nucleotide #: NM_004915
• UniProt Accession #: P45844; Q86SU8; Q96L76; Q9BXK6; Q9BXK7; Q9BXK8; Q9BXK9; Q9BXL0; Q9BXL1; Q9BXL2; Q9BXL3; Q9BXL4
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: ATP-binding cassette sub-family G member 1 isoform 4
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family G (WHITE), member 1
• NCBI Official Symbol: ABCG1
• NCBI Official Synonym Symbols: ABC8; WHITE1
• NCBI Protein Information: ATP-binding cassette sub-family G member 1; ABC transporter 8; ATP-binding cassette transporter 8; ATP-binding cassette transporter member 1 of subfamily G; homolog of Drosophila white; white protein homolog (ATP-binding cassette transporter 8)
• UniProt Protein Name: ATP-binding cassette sub-family G member 1
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG1
• UniProt Synonym Gene Names: ABC8; WHT1
• UniProt Entry Name: ABCG1_HUMAN

Uniprot Description

ABCG1: Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, ABC family; Transporter

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; recycling endosome; mitochondrion; intracellular membrane-bound organelle; integral to plasma membrane; plasma membrane; endosome; external side of plasma membrane

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; phospholipid transporter activity; protein heterodimerization activity; cholesterol transporter activity; cholesterol binding; toxin transporter activity; phospholipid binding; sterol-transporting ATPase activity; ADP binding; ATP binding

Biological Process: cholesterol metabolic process; detection of hormone stimulus; response to high density lipoprotein stimulus; positive regulation of cholesterol biosynthetic process; lipoprotein metabolic process; intracellular cholesterol transport; cholesterol efflux; response to lipid; response to organic substance; cholesterol homeostasis; amyloid precursor protein catabolic process; reverse cholesterol transport; regulation of transcription, DNA-dependent; phospholipid efflux; transmembrane transport; phospholipid homeostasis