Testing Data
(BALB/c mouse splenocytes stained with I-Ad FITC.)
Mouse anti-Mouse MHC Class 2, I-Ad Monoclonal Antibody
MHC Class 2, I-Ad (H-2 Class II Histocompatibility Antigen, I-A beta Chain, H2-Eb1)
Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
MHC Class 2; I-Ad; Monoclonal Antibody; I-Ad (H-2 Class II Histocompatibility Antigen; I-A beta Chain; H2-Eb1); Anti -MHC Class 2; anti-MHC Class 2; I-Ad antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG3,k
Specificity
Recognizes mouse d and g7 haplotype. Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-MHC Class 2, I-Ad antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunofluorescence.
Dilution: Flow Cytometry: 0.25ug/10e6 cells in 100ul volume
Dilution: Flow Cytometry: 0.25ug/10e6 cells in 100ul volume
Immunogen
(C3H x BALB/c)F1 mouse cells
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(BALB/c mouse splenocytes stained with I-Ad FITC.)
Testing Data
(BALB/c mouse splenocytes stained with I-Ad FITC.)
Testing Data
(C57BL/6 mouse splenocytes stained with I-Ad FITC.)
Testing Data
(C57BL/6 mouse splenocytes stained with I-Ad FITC.)
Related Product Information for anti-MHC Class 2, I-Ad antibody
This antibody reacts with the I-Ad MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2d bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. This antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.
Product Categories/Family for anti-MHC Class 2, I-Ad antibody
Similar Products
Product Notes
The MHC Class 2, I-Ad (Catalog #AAA643257) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MHC Class 2, I-Ad (H-2 Class II Histocompatibility Antigen, I-A beta Chain, H2-Eb1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MHC Class 2, I-Ad can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Flow Cytometry, Immunohistochemistry and Immunofluorescence. Dilution: Flow Cytometry: 0.25ug/10e6 cells in 100ul volume. Researchers should empirically determine the suitability of the MHC Class 2, I-Ad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC Class 2, I-Ad, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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