Test Data
(Chicken peripheral blood mononuclear cells were stained with Mouse Anti-Chicken TCRYO-PE (5B Cat. No. MBS673878) and Mouse Anti-Chicken CD3-FITC (5B Cat. No. MBS673862).)
Mouse anti-Chicken TCR gamma delta Monoclonal Antibody
Mouse Anti-Chicken TCRgd-BIOT
Reactivity
Chicken
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
TCR gamma delta; Monoclonal Antibody; Mouse Anti-Chicken TCRgd-BIOT; T3ITCR complex; TCR gamma/delta; anti-TCR gamma delta antibody
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG1K
Clone Number
TCR1
Specificity
Chicken/Milk Snake TCR gamma/delta; Mr 50 & 40 kDa
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for anti-TCR gamma delta antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
Application Notes
Research Application(s): In ovo depletion of gamma delta T cells
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Other Applications: Since application vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Other Applications: Since application vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Immunogen
Outbred chicken thymocytes and Ig-negative blood lymphocytes
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScan flow cytometer.
Preparation and Storage
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Test Data
(Chicken peripheral blood mononuclear cells were stained with Mouse Anti-Chicken TCRYO-PE (5B Cat. No. MBS673878) and Mouse Anti-Chicken CD3-FITC (5B Cat. No. MBS673862).)
Test Data
(Chicken peripheral blood mononuclear cells were stained with Mouse Anti-Chicken TCRYO-PE (5B Cat. No. MBS673878) and Mouse Anti-Chicken CD3-FITC (5B Cat. No. MBS673862).)
Related Product Information for anti-TCR gamma delta antibody
The monoclonal antibody TCR-1 precipitates a heterodimer of Mr 90 kDa (two bands of Mr 50 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 35 kDa and 32 kDa. Unlike human and mouse in which yo cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of yo T cells. The frequency of TCRgamma/delta cells is typically 20-25% of the total blood T cells but may reach approximately 50% in chickens of six- months of age. The majority of TCRgamma/delta+ cells in the thymus and blood are CD4-CD8- although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRgamma/delta+ cells migrate into the spleen and intestine most of them begin to express CD8. Although the biological function of yo T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ yo T cells may also be involved in down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reactions.
References
1. Sowder, J.T., C.-L. Chen, L.L. Ager, M.M. Chan, and M.D. Cooper. 1988. J. Exp. Med. 167:315.
2. Chen, C.H., J. Cihak, U. Losch, and M.D. Cooper. 1988. Eur. J. Immunol. 18:539
3. Quere, P., M.D. Cooper, and G.J. Thorbecke. 1990. Immunology 71:517.
4. Chen, C.H., T.W.F. Gobel, T. Kubota, and M.D. Cooper. 1993. 1994. Poultry Science 73:1012.
5. Chen, C.-L. 1996. Personal communication.
6. Sowder, J.T., C.H. Chen, L.L. Ager, J. Cihak, U. Losch, and M.D. Cooper. 1988b. Fed. Proc. 2:874.
7. Cihak, J., U. Losch, G. Hoffman-Hezer, C.H. Chen, M.D. Cooper, and H.W.L. Ziegler-Heitbrock. 1993. Scand. J. Immunol. 38:123.
2. Chen, C.H., J. Cihak, U. Losch, and M.D. Cooper. 1988. Eur. J. Immunol. 18:539
3. Quere, P., M.D. Cooper, and G.J. Thorbecke. 1990. Immunology 71:517.
4. Chen, C.H., T.W.F. Gobel, T. Kubota, and M.D. Cooper. 1993. 1994. Poultry Science 73:1012.
5. Chen, C.-L. 1996. Personal communication.
6. Sowder, J.T., C.H. Chen, L.L. Ager, J. Cihak, U. Losch, and M.D. Cooper. 1988b. Fed. Proc. 2:874.
7. Cihak, J., U. Losch, G. Hoffman-Hezer, C.H. Chen, M.D. Cooper, and H.W.L. Ziegler-Heitbrock. 1993. Scand. J. Immunol. 38:123.
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Product Notes
The TCR gamma delta (Catalog #AAA673877) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Chicken TCRgd-BIOT reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's TCR gamma delta can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation. Research Application(s): In ovo depletion of gamma delta T cells Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells Other Applications: Since application vary, you should determine the optimum working dilution for the product that is appropriate for your specific need. Researchers should empirically determine the suitability of the TCR gamma delta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR gamma delta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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