Testing Data
Mouse anti-Chicken TCR, alpha, beta, V beta 2 Monoclonal Antibody
TCR, alpha, beta, V beta 2 (T Cell Receptor)
Reactivity
Chicken
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
PurifiedPurified
Synonyms
TCR; alpha; beta; V beta 2; Monoclonal Antibody; V beta 2 (T Cell Receptor); Anti -TCR; anti-TCR; V beta 2 antibody
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
5K83
Specificity
Recognizes chicken TCR alpha, beta. Reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells and 13% of splenocytes young adult chickens.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Applicable Applications for anti-TCR, alpha, beta, V beta 2 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Flow cytometry 1,2, , Immunohistochemistry (acetone-fixed, frozen sections) 3, , In ovo suppression of the development of TCR-Vb2 cells 3,5
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
Testing Data
Related Product Information for anti-TCR, alpha, beta, V beta 2 antibody
This antibody precipitates a CD3-associated heterodimer of Mr 88kD (two bands of Mr 48kD and 40kD upon reduction) on chicken peripheral blood T cells.1,2 Deglycosylation of the heterodimer yields two polypeptides of Mr 34kD and 31kD from antibody precipitates. In the chicken, two distinct subpopulations of alpha/beta T cells appear in the thymus subsequent to the appearance of gamma/delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1,2 arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vb1 and Vb2, respectively.3 Two-color immunofluorescence has revealed that the TCR3+ thymocytes include CD4+CD8-, CD4-CD8+, CD4+CD8+ and CD4-CD8- subpopulations. The TCR3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR3/CD3 complex and most of the cells in this subset are CD4+CD8+. Cells in the other subset express TCR3/CD3 in higher density and are either CD4+CD8- or CD4-CD8+, corresponding to the more mature medullary subset of thymocytes. The TCR3+ cells in the blood and spleen express relatively high levels of the TCR3/CD3 receptor complex and are "single positive, with CD4+CD8- cells being four times more frequent that the CD4-CD8+ cells (ca. 80% CD4+ vs ca. 20% CD8+).1,2
Product Categories/Family for anti-TCR, alpha, beta, V beta 2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
TCR beta chain
Similar Products
Product Notes
The TCR, alpha, beta, V beta 2 (Catalog #AAA632332) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TCR, alpha, beta, V beta 2 (T Cell Receptor) reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's TCR, alpha, beta, V beta 2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Flow cytometry 1,2,, Immunohistochemistry (acetone-fixed, frozen sections) 3,, In ovo suppression of the development of TCR-Vb2 cells 3,5. Researchers should empirically determine the suitability of the TCR, alpha, beta, V beta 2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR, alpha, beta, V beta 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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