Mouse anti-Chicken TCR gamma delta Monoclonal Antibody

Mouse Anti-Chicken TCRgd-FITC

Cat. Num. AAA673876

• Reactivity: Chicken
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: TCR gamma delta; Monoclonal Antibody; Mouse Anti-Chicken TCRgd-FITC; Mouse Anti-Chicken TCRgd - FITC; Mouse Anti-Chicken TCR gamma delta; anti-TCR gamma delta antibody

• Host: Mouse
• Reactivity: Chicken
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: TCR1
• Specificity: Chicken TCR gamma delta ; MAb TCR1 precipitates a heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50-kDa upon reduction) on chicken peripheral blood T cells.1,2 Deglycosylation of the heterodimer yields two polypeptides of Mr 35-kDa and 32-kDa from TCR1 precipitates. Unlike human and mouse, in which gamma delta cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of gamma delta T cells.1-3 The frequency of TCR gamma delta cells is usually 20-25% of the total blood T cells, but may reach approximately 50% in chickens of 6 months of age.2 The majority of TCR gamma delta + cells in the thymus and blood are CD4-CD8-, although a small subset of them may express CD8 or CD4 coreceptors.3 However, when the TCR gamma delta + cells migrate into the spleen and intestine, most of them begin to express CD8. Although the biological function of gamma delta T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ gamma delta T cells may also be involved in down-regulation of the immune response.4 They cannot, however, induce a graft-vs-host (GVH) reactions.5
• Form/Format: Fluorescein (FITC) Conjugate

• Applicable Applications for anti-TCR gamma delta antibody: Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
• Application Notes: Research Application(s): In ovo depletion of gamma delta T cells; ; Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScan flow cytometer.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Testing Data

References

1. Sowder, J.T., C.-L. Chen, L.L. Ager, M.M. Chan, and M.D. Cooper. 1988. J. Exp. Med. 167:315.
2. Chen, C.H., J. Cihak, U. Losch, and M.D. Cooper. 1988. Eur. J. Immunol. 18:539
3. Quere, P., M.D. Cooper, and G.J. Thorbecke. 1990. Immunology 71:517.
4. Chen, C.H., T.W.F. Gobel, T. Kubota, and M.D. Cooper. 1993. 1994. Poultry Science 73:1012.
5. Chen, C.-L. 1996. Personal communication.
6. Sowder, J.T., C.H. Chen, L.L. Ager, J. Cihak, U. Losch, and M.D. Cooper. 1988b. Fed. Proc. 2:874.
7. Cihak, J., U. Losch, G. Hoffman-Hezer, C.H. Chen, M.D. Cooper, and H.W.L. Ziegler-Heitbrock. 1993. Scand. J. Immunol. 38:123.