Flow Cytometry
(Flow Cytometric analysis using TCR antibodyStaining of the DO11.10 T cell hybridoma with 0.125 ug of Mouse IgG2a kappa Isotype Control (open histogram) or 0.125 ug of DO11.10 TCR antibody (filled histogram). Total viable cells were used for analysis.)
Mouse TCR Monoclonal Antibody | anti-TCR antibody
TCR antibody (allophycocyanin)
Applications
Flow Cytometry, Functional Assay
Synonyms
TCR; Monoclonal Antibody; TCR antibody (allophycocyanin); Monoclonal TCR; Anti-TCR; Anti TCR; TCR (APC); anti-TCR antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
KJ1-26
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/ml (varies by lot)
Sequence Length
134
Applicable Applications for anti-TCR antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.06 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested. The TCR expressedon DO11.10 recognizes an ovalbumin peptide in the context of I-A majorhistocompatibility molecules. The use of DO11.10 and KJ1-26 have proven tobe instrumental in describing many of the intricate details of T cellimmunity.
Tag/Conjugate
Allophycocyanin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry
(Flow Cytometric analysis using TCR antibodyStaining of the DO11.10 T cell hybridoma with 0.125 ug of Mouse IgG2a kappa Isotype Control (open histogram) or 0.125 ug of DO11.10 TCR antibody (filled histogram). Total viable cells were used for analysis.)
Flow Cytometry
(Flow Cytometric analysis using TCR antibodyStaining of the DO11.10 T cell hybridoma with 0.125 ug of Mouse IgG2a kappa Isotype Control (open histogram) or 0.125 ug of DO11.10 TCR antibody (filled histogram). Total viable cells were used for analysis.)
Related Product Information for anti-TCR antibody
Mouse monoclonal TCR antibody (allophycocyanin)
Product Categories/Family for anti-TCR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
TCR, partial
Similar Products
Product Notes
The TCR (Catalog #AAA833787) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TCR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.06 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the TCR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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