Flow Cytometry (FC/FACS)
(Staining of mouse splenocytes with TCR beta antibody (FITC) (left), (PE) (right). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.)
Hamster TCR beta Monoclonal Antibody | anti-TCR beta antibody
TCR beta antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
TCR beta; Monoclonal Antibody; TCR beta antibody (biotin); Monoclonal TCR beta; Anti-TCR beta; TCRb; Anti TCR beta; anti-TCR beta antibody
Host
Hamster
Clonality
Monoclonal
Isotype
IgG
Clone Number
H57-597
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
141
Applicable Applications for anti-TCR beta antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to block Fcgamma?receptors with anti?mouse CD16/32 (cat. 14?0161), prior to staining to prevent non?specific binding of eBio3C8. Furthermore, it is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to block Fcgamma?receptors with anti?mouse CD16/32 (cat. 14?0161), prior to staining to prevent non?specific binding of eBio3C8. Furthermore, it is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR beta expressing T cells.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of mouse splenocytes with TCR beta antibody (FITC) (left), (PE) (right). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of mouse splenocytes with TCR beta antibody (FITC) (left), (PE) (right). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.)
Related Product Information for anti-TCR beta antibody
Armenian Hamster monoclonal TCR beta antibody (biotin)
Product Categories/Family for anti-TCR beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
TCR, partial
Similar Products
Product Notes
The TCR beta (Catalog #AAA832347) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TCR beta can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.5 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to block Fcgamma?receptors with anti?mouse CD16/32 (cat. 14?0161), prior to staining to prevent non?specific binding of eBio3C8. Furthermore, it is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the TCR beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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