Typical Testing Data/Standard Curve (for reference only)
Human Hepatocyte Nuclear Factor 6 beta ELISA Kit | HNF6beta elisa kit
Human Hepatocyte Nuclear Factor 6 beta ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This HNF6b enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHNF6b. Standards or samples are then added to the microtiter plate wells andHNF6b if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHNF6b present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHNF6b are added to each well to "sandwich" theHNF6b immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that containHNF6b and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the onecut family of transcription factors, which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes, including genes involved in melanocyte and hepatocyte differentiation. [provided by RefSeq, Jul 2008]
Uniprot Description
ONECUT2: Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B. Belongs to the CUT homeobox family.
Protein type: Transcription factor; Motility/polarity/chemotaxis; Cell development/differentiation; DNA-binding
Chromosomal Location of Human Ortholog: 18q21.31
Cellular Component: nucleoplasm; actin cytoskeleton
Biological Process: peripheral nervous system neuron development; organ morphogenesis; cell fate commitment; transcription, DNA-dependent; regulation of cell-matrix adhesion; cilium biogenesis; epithelial cell development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; cell differentiation; liver development; endocrine pancreas development; positive regulation of cell migration
Research Articles on HNF6beta
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Product Notes
The Human HNF6beta onecut2 (Catalog #AAA720331) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720331 ELISA Kit recognizes Human HNF6beta. It is sometimes possible for the material contained within the vial of "Hepatocyte Nuclear Factor 6 beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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