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Western Blot (WB) (Anti-SOX11 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:58 kDa(We are unsure as to the identity of these extra bands.))

Rabbit SOX11 Polyclonal Antibody | anti-SOX11 antibody

Anti-SOX11 Antibody, Rabbit Polyclonal

Gene Names
SOX11; MRD27
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
SOX11; Polyclonal Antibody; Anti-SOX11 Antibody; Rabbit Polyclonal; SOX11 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-SOX11 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SOX11
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SOX11 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
ICC/IF: 1:1500-1:50,000
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human SOX11
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human SOX11, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SOX11 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:58 kDa(We are unsure as to the identity of these extra bands.))

Western Blot (WB) (Anti-SOX11 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:58 kDa(We are unsure as to the identity of these extra bands.))

Immunofluorescence (IF)

(Immunofluorescence staining of SOX11 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SOX11 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)

Immunofluorescence (IF) (Immunofluorescence staining of SOX11 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SOX11 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
Related Product Information for anti-SOX11 antibody
SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11's transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. SOX11 has emerged as a key transcription factor in the pathogenesis of mantle cell lymphoma (MCL) whereas it is not expressed in normal B cells or virtually in any other mature B-cell neoplasm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46679 MW
NCBI Official Full Name
transcription factor SOX-11
NCBI Official Synonym Full Names
SRY-box 11
NCBI Official Symbol
SOX11
NCBI Official Synonym Symbols
MRD27
NCBI Protein Information
transcription factor SOX-11
UniProt Protein Name
Transcription factor SOX-11
Protein Family
UniProt Gene Name
SOX11

NCBI Description

This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional factor involved in the embryonic neurogenesis. May also have a role in tissue modeling during development.

Research Articles on SOX11

Similar Products

Product Notes

The SOX11 sox11 (Catalog #AAA8101005) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SOX11 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's SOX11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:1000 ICC/IF: 1:1500-1:50,000. Researchers should empirically determine the suitability of the SOX11 sox11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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