Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-TCR antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Mouse anti-Mouse TCR Monoclonal Antibody | anti-TCR antibody
Anti-TCR [Desire-1], Mouse IgG1, Kappa
Reactivity
Mouse
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Protein A affinity purified
Synonyms
TCR; Monoclonal Antibody; Anti-TCR [Desire-1]; Mouse IgG1; Kappa; T cell receptor; T-cell receptor; Ti; anti-TCR antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1, Kappa
Clone Number
Desire-1
Specificity
mAb desire-1 recognises an epitope contributed by both the alpha and beta chains of the KB5-C20 TCR.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TCR antibody
Crystallisation, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Desire-1 has been used for the immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986). An FITC conjugated desire-1 has been used in flow cytometric analysis as a marker for clonotype expression (Schonrich 1991). Further, a Fab fragment derived from desire-1 has been used in the crystallisation of the T-cell receptor for 3D structure determination (Housset 1997).
Antibody Type
Recombinant Antibody
Immunogen
mAb desire-1 was obtained after fusion of spleen cells from a BALB.K mouse immunised twice with KB5-C20 clone cells in RPMI 1640 medium, with non-Ig-producing myeloma cells X63-Ag8.653. Desire-1 was selected for its capacity to inhibit selectively the H-2Kb-specific cytolysis by clone KB5-C20.
Chimeric Use Statement
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-TCR antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-TCR antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-TCR antibody
Describes the ability desire-1 to modulate the cytolytic activity of cytotoxic T lymphocyte clone KB5-C20.
Recombinant monoclonal antibody to TCR. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Desire-1.
Recombinant monoclonal antibody to TCR. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Desire-1.
Product Categories/Family for anti-TCR antibody
References
Hua et al.; Eur. J. Immunol. 1985. 15: 1029-1032
NCBI and Uniprot Product Information
Uniprot Description
MiscellaneousClone B10.A was isolated from a cytotoxic T lymphocyte.
Similar Products
Product Notes
The TCR (Catalog #AAA488504) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-TCR [Desire-1], Mouse IgG1, Kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TCR can be used in a range of immunoassay formats including, but not limited to, Crystallisation, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Desire-1 has been used for the immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986). An FITC conjugated desire-1 has been used in flow cytometric analysis as a marker for clonotype expression (Schonrich 1991). Further, a Fab fragment derived from desire-1 has been used in the crystallisation of the T-cell receptor for 3D structure determination (Housset 1997). Researchers should empirically determine the suitability of the TCR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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