Rabbit AGAP2 Polyclonal Antibody | anti-AGAP2 antibody

AGAP2 (Arf-GAP with GTPase, ANK Repeat and PH Domain-containing Protein 2, AGAP-2, Centaurin-gamma-1, Cnt-g1, GTP-binding and GTPase-activating Protein 2, GGAP2, Phosphatidylinositol-3-kinase Enhancer, PIKE, CENTG1, KIAA0167)

Cat. Num. AAA6010527

• Gene Names: AGAP2; PIKE; GGAP2; CENTG1
• Reactivity: Equine, Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: AGAP2; Polyclonal Antibody; AGAP2 (Arf-GAP with GTPase; ANK Repeat and PH Domain-containing Protein 2; AGAP-2; Centaurin-gamma-1; Cnt-g1; GTP-binding and GTPase-activating Protein 2; GGAP2; Phosphatidylinositol-3-kinase Enhancer; PIKE; CENTG1; KIAA0167); Anti -AGAP2 (Arf-GAP with GTPase; anti-AGAP2 antibody

• Host: Rabbit
• Reactivity: Equine, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AGAP2. Species Crossreactivity: chimpanzee, equine, monkey, mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

• Applicable Applications for anti-AGAP2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-3ug/ml; Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to aa450-500 of human AGAP2.
• Positive Control: Jurkat
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using MBS6010527 at 1ug/ml. goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of AGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and MBS6010527 (bottom left, right) at 5ug/ml.)

Related Product Information for anti-AGAP2 antibody

AGAP2 (also known as PIKE-A), a putative oncogene, shows elevated expression in cancer cells compared to non-transformed cells and has been implicated as a regulator of cancer cell invasion into normal tissues. A contributor to brain, eye, breast and liver cancers, AGAP2 belongs to the centaurin gamma-like family with two ANK repeats, an Arf-GAP domain, a Miro domain and a PH domain. It is a GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. The Arf-GAP domain interacts with the G domain and may regulate its GTPase activity. GAP activity of AGAP2 is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation. Several isoforms of AGAP2 have been reported.

Product Categories/Family for anti-AGAP2 antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 170650694
• NCBI GeneID: 116986
• NCBI Accession #: NP_001116244.1
• NCBI GenBank Nucleotide #: NM_001122772.2
• UniProt Accession #: Q99490; O00578; Q548E0; Q8IWU3; A8K9F7
• Molecular Weight: 124,674 Da
• NCBI Official Full Name: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform PIKE-L
• NCBI Official Synonym Full Names: ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
• NCBI Official Symbol: AGAP2
• NCBI Official Synonym Symbols: PIKE; GGAP2; CENTG1
• NCBI Protein Information: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; cnt-g1; centaurin, gamma 1; phosphoinositide 3-kinase enhancer; phosphatidylinositol 3-kinase enhancer; phosphatidylinositol-3-kinase enhancer; GTP-binding and GTPase activating protein 2; Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2
• UniProt Protein Name: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
• Protein Family: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein
• UniProt Gene Name: AGAP2
• UniProt Synonym Gene Names: CENTG1; KIAA0167; AGAP-2; Cnt-g1; GGAP2; PIKE
• UniProt Entry Name: AGAP2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CENTG1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.

Protein type: GAPs, ARF; Motility/polarity/chemotaxis; GAPs; Tumor suppressor

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: membrane; mitochondrion; nucleolus; nucleus

Molecular Function: protein binding; GTP binding; zinc ion binding

Biological Process: axon guidance; protein transport; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; negative regulation of protein catabolic process; positive regulation of GTPase activity