Rabbit anti-Human AKR1C3 Polyclonal Antibody | anti-AKR1C3 antibody

AKR1C3, ID (AKR1C3, DDH1, HSD17B5, KIAA0119, PGFS, Aldo-keto reductase family 1 member C3, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-HSD type II, brain, 3-alpha-hydroxysteroid dehydrogenase type 2, Chlordecone reductase homolog HAKRb, Dihydrodi

Cat. Num. AAA6272550

• Gene Names: AKR1C3; DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AKR1C3; Polyclonal Antibody; ID (AKR1C3; DDH1; HSD17B5; KIAA0119; PGFS; Aldo-keto reductase family 1 member C3; 17-beta-hydroxysteroid dehydrogenase type 5; 3-alpha-HSD type II; brain; 3-alpha-hydroxysteroid dehydrogenase type 2; Chlordecone reductase homolog HAKRb; Dihydrodi; Dihydrodiol dehydrogenase 3; Dihydrodiol dehydrogenase type I; HA1753; Indanol dehydrogenase; Prostaglandin F synthase; Testosterone 17-beta-dehydrogenase 5; Trans-1; 2-dihydrobenzene-1; 2-diol dehydrogenase; anti-AKR1C3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AKR1C3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-AKR1C3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 107-135 in the central region of human AKR1C3, UniProt Accession #NP_003730.4; P42330.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS646505. This demonstrates that MBS646505 detected the AKR1C3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS646505 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646505 for immunohistochemistry.)

Related Product Information for anti-AKR1C3 antibody

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Product Categories/Family for anti-AKR1C3 antibody

Antibodies; Prostaglandins

References

1. Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884 (2010). 2. Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263 (2010). 3. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 4. Wang, X., et al. PLoS ONE 5 (8), E11934 (2010). 5. Zakharov, V., et al. Int J Clin Exp Pathol 3(6):608-617 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 24497583
• NCBI GeneID: 8644
• NCBI Accession #: NP_003730.4
• NCBI GenBank Nucleotide #: NM_003739.5
• UniProt Accession #: P42330; Q5T2L1; Q96DJ1; Q96KI8; Q99530; Q9UCX1; Q9UII3; Q9UKL9; A8K2V0
• Molecular Weight: 36,853 Da
• NCBI Official Full Name: aldo-keto reductase family 1 member C3 isoform 1
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member C3
• NCBI Official Symbol: AKR1C3
• NCBI Official Synonym Symbols: DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS
• NCBI Protein Information: aldo-keto reductase family 1 member C3; indanol dehydrogenase; prostaglandin F synthase; 3-alpha-HSD type II, brain; dihydrodiol dehydrogenase 3; dihydrodiol dehydrogenase X; chlordecone reductase homolog HAKRb; testosterone 17-beta-dehydrogenase 5; 3-alp
• UniProt Protein Name: Aldo-keto reductase family 1 member C3
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1C3
• UniProt Synonym Gene Names: DDH1; HSD17B5; KIAA0119; PGFS; 17-beta-HSD 5; 3-alpha-HSD type 2; DD-3; DD3; PGFS
• UniProt Entry Name: AK1C3_HUMAN

Uniprot Description

AKR1C3: Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta- PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone. Belongs to the aldo/keto reductase family.

Protein type: Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.1.1.149; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm; intracellular; nucleus; cytosol

Molecular Function: 15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity; delta4-3-oxosteroid 5beta-reductase activity; aldo-keto reductase activity; prostaglandin-F synthase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; retinol dehydrogenase activity; phenanthrene 9,10-monooxygenase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; testosterone 17-beta-dehydrogenase (NADP+) activity; ketosteroid monooxygenase activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; ketoreductase activity; indanol dehydrogenase activity; aldehyde reductase activity; geranylgeranyl reductase activity; retinal dehydrogenase activity

Biological Process: steroid metabolic process; phototransduction, visible light; retinal metabolic process; cyclooxygenase pathway; male gonad development; progesterone metabolic process; cellular response to starvation; prostaglandin metabolic process; keratinocyte differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; protein import into nucleus, translocation; positive regulation of cell proliferation; arachidonic acid metabolic process; multicellular organismal macromolecule metabolic process; farnesol catabolic process; retinoid metabolic process; regulation of retinoic acid receptor signaling pathway; response to nutrient