Western Blot (WB)
(Western Blot (WB) analysis of Bak Rabbit mAb at 1:1000 dilutionLane1:Hela whole cell lysateLane2:The skeletal muscle lysate of HumanLane3:AGS whole cell lysate)
Rabbit anti-Human Bak Monoclonal Antibody | anti-Bak antibody
Bak Rabbit Monoclonal Antibody
IHC: 1:50-1:200
ICC: 1:50-1:200
IF: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Bak Rabbit mAb at 1:1000 dilutionLane1:Hela whole cell lysateLane2:The skeletal muscle lysate of HumanLane3:AGS whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of Bak Rabbit mAb at 1:1000 dilutionLane1:Hela whole cell lysateLane2:The skeletal muscle lysate of HumanLane3:AGS whole cell lysate)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining Bak in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Bak in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Bak antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Bak antibody. Counter stained with hematoxylin.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NIH/3T3 cells with Bak antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NIH/3T3 cells with Bak antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]
Uniprot Description
BAK1: In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. Interacts with BCL2A1. Homodimer. Formation of the homodimer is zinc-dependent. Forms heterodimers with BCL2, E1B 19k protein, and BCL2L1 isoform Bcl-X(L). Interacts with myxoma virus protein M11L. Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. Belongs to the Bcl-2 family.
Protein type: Membrane protein, integral; Apoptosis; Endoplasmic reticulum; Mitochondrial
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: pore complex; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytosol; integral to mitochondrial outer membrane
Molecular Function: identical protein binding; BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity; chaperone binding; heat shock protein binding; metal ion binding
Biological Process: response to fungus; regulation of protein heterodimerization activity; positive regulation of apoptosis; apoptosis; regulation of cell cycle; positive regulation of proteolysis; myeloid cell homeostasis; negative regulation of peptidyl-serine phosphorylation; B cell apoptosis; response to organic cyclic substance; negative regulation of cell proliferation; B cell homeostasis; regulation of mitochondrial membrane potential; response to gamma radiation; establishment and/or maintenance of transmembrane electrochemical gradient; B cell negative selection; aging; response to drug; release of cytochrome c from mitochondria; organ regeneration; mitochondrial fusion; response to mycotoxin; regulation of protein homodimerization activity; vagina development; endocrine pancreas development; limb morphogenesis; response to UV-C; cell proliferation; endoplasmic reticulum calcium ion homeostasis; response to ethanol; unfolded protein response, activation of signaling protein activity; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; caspase activation via cytochrome c; brain development; regulation of mitochondrial membrane permeability; post-embryonic camera-type eye morphogenesis
Research Articles on Bak
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Product Notes
The Bak bak1 (Catalog #AAA3014134) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bak Rabbit Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bak can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 IF: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the Bak bak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bak, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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