Human syntaxin 1A (brain) ELISA Kit | STX1A elisa kit

Human Syntaxin-1A, STX1A ELISA Kit

Cat. Num. AAA912583

• Gene Names: STX1A; STX1; HPC-1; P35-1; SYN1A
• Reactivity: Human
• Synonyms: syntaxin 1A (brain); Human Syntaxin-1A; STX1A ELISA Kit; Human Syntaxin-1A (STX1A) ELISA kit; HPC-1; STX1; p35-1; syntaxin 1A; STX1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human STX1A. No significant cross-reactivity or interference between human STX1A and analogues was observed.
• Sequence Length: 251

• Samples: Serum, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: <3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STX1A elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for STX1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STX1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STX1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STX1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 259906442
• NCBI GeneID: 6804
• NCBI Accession #: NP_001159375.1
• NCBI GenBank Nucleotide #: NM_001165903.1
• UniProt Accession #: Q16623; O15447; O15448; Q12936; Q75MD9; Q7Z5K3; Q9BPZ6
• Molecular Weight: 33,023 Da
• NCBI Official Full Name: syntaxin-1A isoform 2
• NCBI Official Synonym Full Names: syntaxin 1A (brain)
• NCBI Official Symbol: STX1A
• NCBI Official Synonym Symbols: STX1; HPC-1; P35-1; SYN1A
• NCBI Protein Information: syntaxin-1A; neuron-specific antigen HPC-1
• UniProt Protein Name: Syntaxin-1A
• Protein Family: Syntaxin
• UniProt Gene Name: STX1A
• UniProt Synonym Gene Names: STX1
• UniProt Entry Name: STX1A_HUMAN

NCBI Description

This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

STX1A: a type IV membrane protein involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. Three splice variant isoforms have been described.

Protein type: Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: SNARE complex; synaptic vesicle; neuron projection; synaptic vesicle membrane; extracellular region; plasma membrane; integral to membrane; endomembrane system; actomyosin; cytosol; cell junction; secretory granule

Molecular Function: protein binding, bridging; protein domain specific binding; SNAP receptor activity; myosin binding; protein N-terminus binding; calcium-dependent protein binding; ATP-dependent protein binding; SNARE binding; protein binding; chloride channel inhibitor activity; protein heterodimerization activity; myosin head/neck binding; calcium channel inhibitor activity; glycoprotein binding; kinase binding

Biological Process: response to gravity; neurotransmitter secretion; pathogenesis; positive regulation of neurotransmitter secretion; calcium ion-dependent exocytosis; synaptic vesicle fusion to presynaptic membrane; synaptic transmission; intracellular protein transport; vesicle docking; cellular protein metabolic process; glutamate secretion; energy reserve metabolic process; regulation of insulin secretion; positive regulation of exocytosis; synaptic vesicle docking during exocytosis; secretion by cell