Human Syntaxin-1A ELISA Kit | STX1A elisa kit

Human Syntaxin-1A ELISA Kit

Cat. Num. AAA760884

• Gene Names: STX1A; STX1; HPC-1; P35-1; SYN1A
• Reactivity: Human
• Synonyms: Syntaxin-1A; Human Syntaxin-1A ELISA Kit; STX1A/Syntaxin-1A/STX1/HPC-1/P35-1/Neuron-specific antigen HPC-1; STX1A elisa kit

• Reactivity: Human
• Sequence Length: 251

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for STX1A elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human STX1A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human STX1A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human STX1A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human STX1A can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 259906442
• NCBI GeneID: 6804
• NCBI Accession #: NP_001159375.1
• NCBI GenBank Nucleotide #: NM_001165903.1
• Molecular Weight: 28,995 Da
• NCBI Official Full Name: syntaxin-1A isoform 2
• NCBI Official Synonym Full Names: syntaxin 1A
• NCBI Official Symbol: STX1A
• NCBI Official Synonym Symbols: STX1; HPC-1; P35-1; SYN1A
• NCBI Protein Information: syntaxin-1A
• UniProt Protein Name: Syntaxin-1A
• Protein Family: Syntaxin
• UniProt Gene Name: STX1A
• UniProt Synonym Gene Names: STX1
• UniProt Entry Name: STX1A_HUMAN

NCBI Description

This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

STX1A: a type IV membrane protein involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. Three splice variant isoforms have been described.

Protein type: Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytosol; integral to membrane; neuron projection; plasma membrane; SNARE complex; synaptic vesicle; voltage-gated potassium channel complex

Molecular Function: chloride channel inhibitor activity; kinase binding; myosin binding; protein binding; SNAP receptor activity; SNARE binding

Biological Process: glutamate secretion; intracellular protein transport; neurotransmitter secretion; positive regulation of calcium ion-dependent exocytosis; positive regulation of catecholamine secretion; regulation of insulin secretion; secretion by cell; synaptic vesicle fusion to presynaptic membrane; vesicle docking