Human Syntaxin-1A ELISA Kit | STX1A elisa kit

Human Syntaxin-1A ELISA Kit

Cat. Num. AAA288837

• Gene Names: STX1A; STX1; HPC-1; P35-1; SYN1A
• Reactivity: Human
• Synonyms: Syntaxin-1A; Human Syntaxin-1A ELISA Kit; Neuron-specific antigen HPC-1; STX1A; STX1; STX1A elisa kit

• Reactivity: Human
• Sequence Length: 251

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.098ng/mL
• Intra-Assay CV: <=4.7%
• Inter-Assay CV: <=7.5%
• Recovery: 103%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STX1A elisa kit

Intended Uses||This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 259906442
• NCBI GeneID: 6804
• NCBI Accession #: NP_001159375.1
• NCBI GenBank Nucleotide #: NM_001165903.1
• UniProt Accession #: Q16623; O15447; O15448; Q12936; Q75MD9; Q7Z5K3; Q9BPZ6
• Molecular Weight: 28,995 Da
• NCBI Official Full Name: syntaxin-1A isoform 2
• NCBI Official Synonym Full Names: syntaxin 1A
• NCBI Official Symbol: STX1A
• NCBI Official Synonym Symbols: STX1; HPC-1; P35-1; SYN1A
• NCBI Protein Information: syntaxin-1A
• UniProt Protein Name: Syntaxin-1A
• Protein Family: Syntaxin
• UniProt Gene Name: STX1A
• UniProt Synonym Gene Names: STX1
• UniProt Entry Name: STX1A_HUMAN

NCBI Description

This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

Plays a role in hormone and neurotransmitter exocytosis (). Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.