Mouse Synaptobrevin-2 Monoclonal Antibody | anti-VAMP2 antibody

Mouse Anti Human Synaptobrevin-2

Cat. Num. AAA140315

• Gene Names: VAMP2; SYB2; VAMP-2
• Applications: ELISA, Western Blot
• Purity: VAMP2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Synaptobrevin-2; Monoclonal Antibody; Mouse Anti Human Synaptobrevin-2; VAMP2 Antibody; Mouse Anti Human; Vesicle-associated membrane protein 2; SYB2; VAMP-2; VAMP2; FLJ11460; anti-VAMP2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain and kappa light chain
• Clone Number: P3E5AT
• Purity/Purification: VAMP2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 116

• Applicable Applications for anti-VAMP2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: VAMP2 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.
• Immunogen: Anti-human VAMP2 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human VAMP2 amino acids 1-89 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-VAMP2 antibody

Introduction: Synaptobrevin 2 which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25.

Product Categories/Family for anti-VAMP2 antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 172072620
• NCBI GeneID: 6844
• NCBI Accession #: NP_055047.2
• NCBI GenBank Nucleotide #: NM_014232.2
• Molecular Weight: 12,663 Da
• NCBI Official Full Name: vesicle-associated membrane protein 2
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 2 (synaptobrevin 2)
• NCBI Official Symbol: VAMP2
• NCBI Official Synonym Symbols: SYB2; VAMP-2
• NCBI Protein Information: vesicle-associated membrane protein 2; synaptobrevin 2; synaptobrevin-2; vesicle associated membrane protein 2
• UniProt Protein Name: Vesicle-associated membrane protein 2
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP2
• UniProt Synonym Gene Names: SYB2; VAMP-2
• UniProt Entry Name: VAMP2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008]

Uniprot Description

VAMP2: a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin.

Protein type: Vesicle; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: neuron projection; zymogen granule membrane; synaptic vesicle membrane; clathrin-coated vesicle; intracellular membrane-bound organelle; integral to plasma membrane; secretory granule membrane; trans-Golgi network; cytosol; secretory granule; SNARE complex; synaptic vesicle; membrane; perinuclear region of cytoplasm; plasma membrane; synapse; cytoplasmic vesicle; cell junction

Molecular Function: calmodulin binding; SNAP receptor activity; protein binding; SNARE binding; protein self-association; syntaxin binding; syntaxin-1 binding; phospholipid binding; calcium-dependent protein binding

Biological Process: exocytosis; natural killer cell degranulation; neurotransmitter secretion; neutrophil degranulation; pathogenesis; calcium ion-dependent exocytosis; vesicle-mediated transport; regulation of exocytosis; vesicle fusion; synaptic transmission; protein transport; synaptic vesicle exocytosis; cellular protein metabolic process; glutamate secretion; cellular response to insulin stimulus; Golgi to plasma membrane protein transport; response to glucose stimulus; energy reserve metabolic process; eosinophil degranulation; protein complex assembly; post-Golgi vesicle-mediated transport; regulation of insulin secretion