VAMP2 recombinant protein

VAMP2, 1-94aa, Human, His-tag, E Coli

Cat. Num. AAA206686

• Gene Names: VAMP2; SYB2; VAMP-2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: VAMP2; 1-94aa; Human; His-tag; E Coli; Vesicle-associated membrane protein 2 isoform 1; VAMP-2; SYB2; VAMP2 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in phosphate buffered saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSATAA TAPPAAPAGE GGPPAPPPNL TSNRRLQQTQ AQVDEVVDIM RVNVDKVLER DQKLSELDDR ADALQAGASQ FETSAAKLKR KYWWKNLK
• Sequence Length: 116

• Applicable Applications for VAMP2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for VAMP2 recombinant protein

VAMP2, also known as vehicle-associated membrane protein 2 isoform 1, which is an integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. VAMP2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. Recombinant human VAMP2 protein, fused to His-tag at N-terminus, was expressed in E,coli and using conventional chromatography.

Product Categories/Family for VAMP2 recombinant protein

Neuroscience; Transportation

References

Lin RC., et al. (1997) Neuron. 19 (5):1087-94.; Hanson PI., et al. (1997) Cell. 90 (3):523-35.; Scales SJ., et al. (2002) J Biol Chem. 277 (31):28271-9.; Windoffer R., et al. (1999) Cell Tissue Res. 296 (3):499-510.

NCBI and Uniprot Product Information

• NCBI GI #: 172072620
• NCBI GeneID: 6844
• NCBI Accession #: NP_055047
• NCBI GenBank Nucleotide #: NM_014232.2
• UniProt Accession #: P63027; P19065; Q9BUC2
• Molecular Weight: 12.8 kDa (118aa), confirmed by MALDI-TOF
• NCBI Official Full Name: vesicle-associated membrane protein 2 isoform 1
• NCBI Official Synonym Full Names: vesicle associated membrane protein 2
• NCBI Official Symbol: VAMP2
• NCBI Official Synonym Symbols: SYB2; VAMP-2
• NCBI Protein Information: vesicle-associated membrane protein 2
• UniProt Protein Name: Vesicle-associated membrane protein 2
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP2
• UniProt Synonym Gene Names: SYB2; VAMP-2

NCBI Description

The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the targeting and/or fusion of transport vesicles to their target membrane. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.