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Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-3: Human fetal brain tissue, Mouse brain tissue, Hela cells, Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)

Rabbit anti-Human, Mouse VAMP2 Polyclonal Antibody | anti-VAMP2 antibody

VAMP2 Antibody

Gene Names
VAMP2; SYB2; VAMP-2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
VAMP2; Polyclonal Antibody; VAMP2 Antibody; SYB2; VAMP-2; anti-VAMP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total VAMP2 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.3 mg/ml (varies by lot)
Sequence Length
116
Applicable Applications for anti-VAMP2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:30-1:150
Immunogen
Synthetic peptide of human VAMP2
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-3: Human fetal brain tissue, Mouse brain tissue, Hela cells, Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)

Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-3: Human fetal brain tissue, Mouse brain tissue, Hela cells, Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/20.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/20.)
Related Product Information for anti-VAMP2 antibody
The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.
Product Categories/Family for anti-VAMP2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,663 Da
NCBI Official Full Name
vesicle-associated membrane protein 2
NCBI Official Synonym Full Names
vesicle associated membrane protein 2
NCBI Official Symbol
VAMP2
NCBI Official Synonym Symbols
SYB2; VAMP-2
NCBI Protein Information
vesicle-associated membrane protein 2
UniProt Protein Name
Vesicle-associated membrane protein 2
UniProt Gene Name
VAMP2
UniProt Synonym Gene Names
SYB2; VAMP-2
UniProt Entry Name
VAMP2_HUMAN

Uniprot Description

VAMP2: a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: clathrin-coated vesicle; cytoplasmic vesicle; cytosol; integral to plasma membrane; intracellular membrane-bound organelle; membrane; neuron projection; perinuclear region of cytoplasm; plasma membrane; secretory granule; secretory granule membrane; SNARE complex; synapse; synaptic vesicle; synaptic vesicle membrane; trans-Golgi network; vesicle; voltage-gated potassium channel complex; zymogen granule membrane

Molecular Function: calcium-dependent protein binding; calmodulin binding; phospholipid binding; protein binding; protein self-association; SNAP receptor activity; SNARE binding; syntaxin binding; syntaxin-1 binding

Biological Process: calcium ion-dependent exocytosis; cellular response to insulin stimulus; eosinophil degranulation; exocytosis; glutamate secretion; Golgi to plasma membrane protein transport; natural killer cell degranulation; neurotransmitter secretion; neutrophil degranulation; post-Golgi vesicle-mediated transport; protein complex assembly; protein transport; regulation of exocytosis; response to glucose stimulus; synaptic vesicle exocytosis; vesicle fusion; vesicle-mediated transport

Similar Products

Product Notes

The VAMP2 vamp2 (Catalog #AAA9415348) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VAMP2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VAMP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:30-1:150. Researchers should empirically determine the suitability of the VAMP2 vamp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAMP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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