Immunofluorescence (IF)
(Immunofluorescence staining of fixed HEK293 cells with anti-SUV420H2 antibody RAB-C409 (MBS488830) Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RAB-C409 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Rabbit anti-Human SUV420H2 Monoclonal Antibody | anti-SUV420H2 antibody
Anti-SUV420H2 [RAB-C409]
Immunofluorescence (IF)
(Immunofluorescence staining of fixed HEK293 cells with anti-SUV420H2 antibody RAB-C409 (MBS488830) Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RAB-C409 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed HEK293 cells with anti-SUV420H2 antibody RAB-C409 (MBS488830) Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RAB-C409 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the anti-SUV420H2 antibody RAB-C409 (MBS488830). HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG1 version of RAB-C409 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the anti-SUV420H2 antibody RAB-C409 (MBS488830). HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG1 version of RAB-C409 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
This antibody was obtained by recombinant antibody (rAb) phage display recognizing SUV420H2 protein under non-denaturing conditions.
Note on publication: More information on this antibody can be found on the website of the Recombinant Antibody Network.
NCBI and Uniprot Product Information
Uniprot Description
SUV420H2: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine
Chromosomal Location of Human Ortholog: 19q13.42
Cellular Component: nucleoplasm; centric heterochromatin; nuclear heterochromatin; condensed nuclear chromosome, pericentric region
Molecular Function: protein binding; histone lysine N-methyltransferase activity (H4-K20 specific)
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Product Notes
The SUV420H2 suv420h2 (Catalog #AAA488831) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SUV420H2 [RAB-C409] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SUV420H2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation-Mass Spectrometry (IP-MS), Chromatin Immunoprecipitation (ChIP). This antibody has been used and recommended for the following methods: ELISA; IP-MS; ChIP. Researchers should empirically determine the suitability of the SUV420H2 suv420h2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUV420H2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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