Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Basic helix-loop-helix transcription factor scleraxis (SCXA) Standard Curve.)
Human Basic Helix-Loop-Helix Transcription Factor Scleraxis (SCXA) ELISA Kit | SCXA elisa kit
Human Basic Helix-Loop-Helix Transcription Factor Scleraxis (SCXA) ELISA Kit
Gene Names
SCX; SCXA; SCXB; bHLHa48
Reactivity
Human
Synonyms
Basic Helix-Loop-Helix Transcription Factor Scleraxis (SCXA); Human Basic Helix-Loop-Helix Transcription Factor Scleraxis (SCXA) ELISA Kit; SCXA; SCX; bHLHa41; class bHLH protein scleraxis; scleraxis homolog A; SCXA elisa kit
Reactivity
Human
Specificity
Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCXA. No significant cross-reactivity or interference between Human SCXA and analogues was observed.
Sequence Length
201
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Basic helix-loop-helix transcription factor scleraxis (SCXA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Basic helix-loop-helix transcription factor scleraxis (SCXA) Standard Curve.)
Related Product Information for SCXA elisa kit
Description: This Human Basic helix-loop-helix transcription factor scleraxis (SCXA) ELISA Kit employs a two-site sandwich ELISA to quantitate SCXA in samples. An antibody specific for SCXA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCXA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCXA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCXA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: By real-time PCR, Schulze-Tanzil et al. (2004) found that expression of SCX, a tendon-specific marker, increased in human tenocytes during their entire time in high-density cultures.By in situ hybridization, Brent et al. (2003) found that Scx was expressed in axial and ventrolateral body wall tendons during chicken embryonic development. It was also expressed in a population of tendon progenitors in developing somites. Brent et al. (2003) determined that FGF signaling in the myotome was both necessary and sufficient for induction of Scx expression in developing chicken somites. Overexpression of Pax1 inhibited Scx expression in somites.Hartz (2004) mapped the SCX gene to chromosome 8q24.3 based on an alignment of the SCX sequence with the genomic sequence.
Background: By real-time PCR, Schulze-Tanzil et al. (2004) found that expression of SCX, a tendon-specific marker, increased in human tenocytes during their entire time in high-density cultures.By in situ hybridization, Brent et al. (2003) found that Scx was expressed in axial and ventrolateral body wall tendons during chicken embryonic development. It was also expressed in a population of tendon progenitors in developing somites. Brent et al. (2003) determined that FGF signaling in the myotome was both necessary and sufficient for induction of Scx expression in developing chicken somites. Overexpression of Pax1 inhibited Scx expression in somites.Hartz (2004) mapped the SCX gene to chromosome 8q24.3 based on an alignment of the SCX sequence with the genomic sequence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
basic helix-loop-helix transcription factor scleraxis
NCBI Official Synonym Full Names
scleraxis bHLH transcription factor
NCBI Official Symbol
SCX
NCBI Official Synonym Symbols
SCXA; SCXB; bHLHa48
NCBI Protein Information
basic helix-loop-helix transcription factor scleraxis
UniProt Protein Name
Basic helix-loop-helix transcription factor scleraxis
UniProt Gene Name
SCX
UniProt Synonym Gene Names
BHLHA41; BHLHA48; SCXA; SCXB; bHLHa41; bHLHa48
Uniprot Description
Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
Research Articles on SCXA
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Product Notes
The Human SCXA scx (Catalog #AAA9714397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714397 ELISA Kit recognizes Human SCXA. It is sometimes possible for the material contained within the vial of "Basic Helix-Loop-Helix Transcription Factor Scleraxis (SCXA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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