Human anti-Human PRMT5 Monoclonal Antibody | anti-PRMT5 antibody

Anti-PRMT5 [RAB-C136]

Cat. Num. AAA488810

• Gene Names: PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Purified antibody. Purified by Immobilized Metal Affinity Chromatography
• Synonyms: PRMT5; Monoclonal Antibody; Anti-PRMT5 [RAB-C136]; Protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; SKB1 homolog; SKB1Hs; anti-PRMT5-RAB-C136; 48F3_10; anti-PRMT5 antibody

• Host: Human
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Human Fab fragment, kappa
• Clone Number: RAB-C136
• Specificity: This antibody recognizes PRMT5 (Protein arginine N-methyltransferase 5). It binds to a folded domain, amino acids 269-637. PRMT5 is a arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA.
• Purity/Purification: Purified antibody. Purified by Immobilized Metal Affinity Chromatography
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PRMT5 antibody: ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation-Mass Spectrometry (IP-MS), Chromatin Immunoprecipitation (ChIP)
• Application Notes: This antibody has been used and recommended for the following methods: ELISA; IF; IP-MS; ChIP.
• Heavy Chain Modification: His-Tagged
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed HeLa cells with anti-PRMT5 antibody RAB-C136 (MBS488810) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RAB-C136 (MBS488811) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488811, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometry using the anti-PRMT5 antibody RAB-C136 (MBS488810). HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG1 version of RAB-C136 (MBS488811, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

Related Product Information for anti-PRMT5 antibody

Recombinant monoclonal antibody to PRMT5. Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody RAB-C136.
This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRMT5 protein under non-denaturing conditions.

Product Categories/Family for anti-PRMT5 antibody

immunology; cancer; cell biology

References

N/A
Note on publication: More information on this antibody can be found on the website of the Recombinant Antibody Network.

NCBI and Uniprot Product Information

• NCBI GI #: 32171585
• NCBI GeneID: 10419
• UniProt Accession #: O14744; Q6IBR1; Q9UKH1; A8MTP3; A8MZ91; B4DX49; B4DY30; B5BU10; D3DS33; E2QRE7
• Molecular Weight: 637
• NCBI Official Full Name: Protein arginine N-methyltransferase 5
• NCBI Official Synonym Full Names: protein arginine methyltransferase 5
• NCBI Official Symbol: PRMT5
• NCBI Official Synonym Symbols: JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• NCBI Protein Information: protein arginine N-methyltransferase 5; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT5
• UniProt Synonym Gene Names: HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
• UniProt Entry Name: ANM5_HUMAN

NCBI Description

This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

PRMT5: an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.

Protein type: EC 2.1.1.125; Cell cycle regulation; Methyltransferase, protein arginine; RNA processing; Methyltransferase

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: methyltransferase activity; ribonucleoprotein binding; protein binding; protein-arginine omega-N symmetric methyltransferase activity; histone-arginine N-methyltransferase activity; chromatin binding; transcription corepressor activity

Biological Process: cell proliferation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; peptidyl-arginine methylation; spliceosomal snRNP biogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of mitosis; gene expression; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; peptidyl-arginine N-methylation; endothelial cell activation