Mouse anti-Rat Surfactant D Monoclonal Antibody
Surfactant Protein D antibody
Reactivity
Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
IgG1 purified from tissue culture supernatant
Synonyms
Surfactant D; Monoclonal Antibody; Surfactant Protein D antibody; Monoclonal Surfactant Protein D; Anti-Surfactant Protein D; Surfactant Protein D; Anti-Surfactant D; SP-D; anti-Surfactant D antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG1kappa
Clone Number
IVF11
Specificity
Rat, Human (recognizes an epitope localized in the lectin domain of rat surfactant protein D)
Purity/Purification
IgG1 purified from tissue culture supernatant
Form/Format
Lyophilized from buffer containing 5 mg/ml BSA and 0.09% sodium azide
Applicable Applications for anti-Surfactant D antibody
Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB), ELISA (EIA)
Application Notes
IHC: fresh rat tissue 1:400; fresh human tissue 1:20. Fix in acetone for 5 min at 4 deg, paraformaldehyde, paraffin. Pretreat paraffin tissues in microwave for 2 x 5 min at 750 W.
ELISA: Use as a capture antibody.
ELISA: Use as a capture antibody.
Biological Significance
Surfactant protein D is acalcium dependent carbohydrate binding protein. The function of SP-D is not fully identified butit is known that SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A. It has a similar structure to other C-type mammalian lectins and a molecular weightof 620 kDa (non-reducing conditions) and 43 kDa (reducing conditions).
Cross-Reactivity
Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells, SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells are negative. No reaction on rat skin, colon, kidney or liver. SP-A cross reacitivity is typically evident at very low dilutions.
Immunogen
Surfactant Protein D antibody was raised using purified rat SP-D from bronchoalveolar lavage
Preparation and Storage
Store lyophilized material at 2-8 degree for up to 1 year. Reconstitute with 500 ul sterile distilled water, aliquot and store at -20 degree for up to 6 months, avoiding repeated freeze/thaw cycles.
Related Product Information for anti-Surfactant D antibody
Mouse monoclonal Surfactant Protein D antibody
Product Categories/Family for anti-Surfactant D antibody
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Product Notes
The Surfactant D (Catalog #AAA832777) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Surfactant Protein D antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Surfactant D can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB), ELISA (EIA). IHC: fresh rat tissue 1:400; fresh human tissue 1:20. Fix in acetone for 5 min at 4 deg, paraformaldehyde, paraffin. Pretreat paraffin tissues in microwave for 2 x 5 min at 750 W. ELISA: Use as a capture antibody. Researchers should empirically determine the suitability of the Surfactant D for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Surfactant D, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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