Chromatin Immunoprecipitation (ChIP)
(Supt5H/SPT5 antibody (mAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of K562 cell chromatin and 4 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 12 million sequence tags were mapped to identify Supt5H binding sites on chromosome 19.)
Rat anti-Human Supt5H/SPT5 Monoclonal Antibody | anti-Supt5H/SPT5 antibody
Supt5H/SPT5 antibody (mAb)(Clone 6F1)
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Protein G Chromatography
Synonyms
Supt5H/SPT5; Monoclonal Antibody; Supt5H/SPT5 antibody (mAb)(Clone 6F1); SPT5 Homolog; DSIF Elongation Factor Subunit; DSIF Large Subunit; Transcription Elongation Factor SPT5; anti-Supt5H/SPT5 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6F1
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Supt5H/SPT5 antibody
Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
ChIP-Seq: 4ug per ChIP
WB: 0.5-2ug/ml
IP: 5ug/ml
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
ChIP-Seq: 4ug per ChIP
WB: 0.5-2ug/ml
IP: 5ug/ml
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
Immunogen
This antibody was raised against a synthetic peptide derived from the human SPT5 C-terminal region.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Supt5H/SPT5 antibody (mAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of K562 cell chromatin and 4 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 12 million sequence tags were mapped to identify Supt5H binding sites on chromosome 19.)
Chromatin Immunoprecipitation (ChIP)
(Supt5H/SPT5 antibody (mAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of K562 cell chromatin and 4 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 12 million sequence tags were mapped to identify Supt5H binding sites on chromosome 19.)
Western Blot (WB)
(Supt5H/SPT5 antibody (mAb) tested by Immunoprecipitation 5 ug of Supt5H antibody (Lane 1) or an unrelated IgG (Lane 2) was used to immunoprecipitate Supt5H from 300 ug of K562 nuclear extract. The immunoprecipitated protein was detected by Western blotting using a the same Supt5H antibody at 2 ug/ml (crossreaction between heavy and light chains of the antibody is observed and is common).)
Western Blot (WB)
(Supt5H/SPT5 antibody (mAb) tested by Immunoprecipitation 5 ug of Supt5H antibody (Lane 1) or an unrelated IgG (Lane 2) was used to immunoprecipitate Supt5H from 300 ug of K562 nuclear extract. The immunoprecipitated protein was detected by Western blotting using a the same Supt5H antibody at 2 ug/ml (crossreaction between heavy and light chains of the antibody is observed and is common).)
Western Blot (WB)
(Supt5H/SPT5 antibody (mAb) tested by Western blot 20 ug of K562 nuclear extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
Western Blot (WB)
(Supt5H/SPT5 antibody (mAb) tested by Western blot 20 ug of K562 nuclear extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
Related Product Information for anti-Supt5H/SPT5 antibody
Short Description: Our Supt5H/SPT5 antibody (mAb)(Clone 6F1) was raised in a Rat host. It has been validated for use in ChIP-Seq, Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: SUPT5H (SPT5 Homolog, DSIF Elongation Factor Subunit) is a transcription elongation factor which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. SUPT5H also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Diseases associated with SUPT5H include HIV-1. Among its related pathways are mRNA capping and HIV life cycle.
Background: SUPT5H (SPT5 Homolog, DSIF Elongation Factor Subunit) is a transcription elongation factor which regulates mRNA processing and transcription elongation by RNA polymerase II. SUPT5H positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. SUPT5H also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Diseases associated with SUPT5H include HIV-1. Among its related pathways are mRNA capping and HIV life cycle.
Product Categories/Family for anti-Supt5H/SPT5 antibody
NCBI and Uniprot Product Information
Molecular Weight
140kDa
Similar Products
Product Notes
The Supt5H/SPT5 (Catalog #AAA389058) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Supt5H/SPT5 antibody (mAb)(Clone 6F1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Supt5H/SPT5 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: ChIP-Seq: 4ug per ChIP WB: 0.5-2ug/ml IP: 5ug/ml Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody. Researchers should empirically determine the suitability of the Supt5H/SPT5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Supt5H/SPT5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
