Mouse anti-Human STK17B / DRAK2 Monoclonal Antibody | anti-STK17B antibody

Mouse Monoclonal [clone 2C3] (IgG2b,k) to Human STK17B / DRAK2

Cat. Num. AAA249158

• Gene Names: STK17B; DRAK2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: STK17B / DRAK2; Monoclonal Antibody; Mouse Monoclonal [clone 2C3] (IgG2b; k) to Human STK17B / DRAK2; Anti-STK17B / DRAK2 Antibody (clone 2C3) IHC-plus; STK17B; DRAK2; Serine/threonine kinase 17b; Human STK17B; anti-STK17B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2C3
• Specificity: Human DRAK2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.37 mg/ml (varies by lot)
• Sequence Length: 372

• Applicable Applications for anti-STK17B antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (3 - 5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: STK17B (AAH16040, 272 a.a. ~ 372 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: STK17B / DRAK2 antibody was raised against sTK17B (AAH16040, 272 a.a. ~ 372 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-STK17B / DRAK2 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to STK17B on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(STK17B monoclonal antibody (M01), clone 2C3 Western blot of STK17B expression in Jurkat.)

ELISA (EIA)

(Detection limit for recombinant GST tagged STK17B is approximately 0.3 ng/ml as a capture antibody.)

Related Product Information for anti-STK17B antibody

STK17B, a DAPK type protein kinase, functions in apoptotic signaling. In vitro assays have shown that protein is autophosphorylated. Messanger RNAs of various in sizes have been reported, likely due to differences of 3' untranslated region.

Product Categories/Family for anti-STK17B antibody

Family: Protein Kinase; Subfamily: DAPK

NCBI and Uniprot Product Information

• NCBI GI #: 4758194
• NCBI GeneID: 9262
• NCBI Accession #: NP_004217.1
• NCBI GenBank Nucleotide #: NM_004226.3
• UniProt Accession #: O94768
• Molecular Weight: 42,344 Da
• NCBI Official Full Name: serine/threonine-protein kinase 17B
• NCBI Official Synonym Full Names: serine/threonine kinase 17b
• NCBI Official Symbol: STK17B
• NCBI Official Synonym Symbols: DRAK2
• NCBI Protein Information: serine/threonine-protein kinase 17B; death-associated protein kinase-related 2; DAP kinase-related apoptosis-inducing protein kinase 2
• UniProt Protein Name: Serine/threonine-protein kinase 17B
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK17B
• UniProt Synonym Gene Names: DRAK2
• UniProt Entry Name: ST17B_HUMAN

Uniprot Description

DRAK2: a calmodulin-dependent kinase of the DAPK family. Interacts with and is regulated by calcineurin homologous protein (CHP), an EF-hand Ca(2+)-binding protein. In vitro, CHP inhibits the apoptosis-inducing protein kinase DRAK2 in the presence of elevated Ca(2+) levels. Highly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 2q32.3

Cellular Component: ER-Golgi intermediate compartment; plasma membrane; nucleus; actin cytoskeleton

Molecular Function: protein serine/threonine kinase activity; ATP binding; protein kinase activity

Biological Process: apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation