SNAP-25 (cleaved & uncleaved) Monoclonal Antibody | anti-SNAP25 antibody
Anti-SNAP-25 Monoclonal Antibody 5G4-1D1
Antibody
Specifity
% Cross Reactivity: 100
Polypeptide: SNAP-25 (cleaved by BoTox-A)
% Cross Reactivity: 100
Polypeptide: VAMP 2
% Cross Reactivity: 0
Polypeptide: Syntaxin 1
% Cross Reactivity: 0
For long term storage, store frozen at -20°C or colder.
Avoid repeated freeze/thaw cycles.
NCBI and Uniprot Product Information
NCBI Description
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]
Uniprot Description
Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Subunit structure: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with CENPF, TRIM9, RIMS1, SNAPIN, OTOF and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1
Subcellular location: Cytoplasm › perinuclear region
By similarity. Cell membrane; Lipid-anchor. Cell junction › synapse › synaptosome. Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region
Tissue specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Post-translational modification: Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association
Miscellaneous: When cloned and expressed in Eschericia coli, where protein palmitoylation does not occur, Cys-85, Cys-88, Cys-90 and Cys-92 in the protein sequence readily form an iron-sulfur cluster instead.
Sequence similarities: Belongs to the SNAP-25 family.Contains 2 t-SNARE coiled-coil homology domains.
Research Articles on SNAP25
Similar Products
Product Notes
The SNAP25 snap25 (Catalog #AAA350063) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SNAP-25 (cleaved & uncleaved) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemical Staining of Rat Brain Section. This monoclonal antibody containing culture supernatant has been found to stain fixed normal rat brain sections in cells known to express SNAP-25 at a dilution of 1:100. The immunocytochemical staining was observed in puncta and at synaptic terminals. Researchers should empirically determine the suitability of the SNAP25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNAP-25 (cleaved & uncleaved), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
