Goat TPD52L2/D54 Polyclonal Antibody | anti-TPD52L2 antibody

Goat anti-TPD52L2/D54 Antibody

Cat. Num. AAA420564

• Gene Names: TPD52L2; D54; TPD54
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TPD52L2/D54; Polyclonal Antibody; Goat anti-TPD52L2/D54 Antibody; TPD52L2; D54; tumor protein D52-like 2; DKFZp686A1765; hD54; HCCR-binding protein 2; TPD52L2 antibody; D54 antibody; tumor protein D52-like 2 antibody; DKFZp686A1765 antibody; hD54 antibody; HCCR-binding protein 2 antibody; TPD52L2 / D54; anti-TPD52L2 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SGDKPLSDPAP
• Sequence Length: 229

• Applicable Applications for anti-TPD52L2 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Approx 28kDa band observed in Human Breast Cancer and Human Brain lysates (calculated MW of 24.9kDa according to NP_955392.1). Recommended concentration: 0.1-0.3ug/ml.
• Immunogen: Peptide with sequence C-SGDKPLSDPAP, from the C Terminus of the protein sequence according to NP_955392.1; NP_955393.1; NP_955394.1; NP_955395.1; NP_003279.2; NP_955391.1.
• Epitope: C Terminus
• Note: This antibody is expected to recognise all reported isoforms (NP_955392.1; NP_955393.1; NP_955394.1; NP_955395.1; NP_003279.2; NP_955391.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 40805864
• NCBI GeneID: 7165
• NCBI Accession #: NP_955392.1; NP_955393.1; NP_955394.1; NP_955395.1; NP_003279.2; NP_955391.1
• NCBI GenBank Nucleotide #: NM_199360.2
• Molecular Weight: 24,854 Da
• NCBI Official Full Name: tumor protein D54 isoform a
• NCBI Official Synonym Full Names: tumor protein D52 like 2
• NCBI Official Symbol: TPD52L2
• NCBI Official Synonym Symbols: D54; TPD54
• NCBI Protein Information: tumor protein D54
• UniProt Protein Name: Tumor protein D54
• Protein Family: Tumor protein
• UniProt Gene Name: TPD52L2
• UniProt Synonym Gene Names: hD54
• UniProt Entry Name: TPD54_HUMAN

NCBI Description

This gene encodes a member of the tumor protein D52-like family. These proteins are characterized by an N-terminal coiled-coil motif that is used to form homo- and heteromeric complexes with other tumor protein D52-like proteins. Expression of this gene may be a marker for breast cancer and acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 12. [provided by RefSeq, Aug 2011]

Uniprot Description

TPD52L2: a cell cycle-regulated protein maximally expressed at the G2-M transition in breast cancer cells. Two alternatively sp[liced isoforms have been reported.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 20q13.2-q13.3