Mouse anti-Human, Mouse SMURF1 Monoclonal Antibody | anti-SMURF1 antibody

Mouse Monoclonal [clone 1D7] (IgG2a,k) to Human SMURF1

Cat. Num. AAA245616

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation, ELISA
• Purity: Protein A Purified
• Synonyms: SMURF1; Monoclonal Antibody; Mouse Monoclonal [clone 1D7] (IgG2a; k) to Human SMURF1; Anti-SMURF1 Antibody (clone 1D7) IHC-plus; E3 ubiquitin ligase SMURF1; KIAA1625; HSMURF1; Human SMURF1; anti-SMURF1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1D7
• Specificity: Human SMURF1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4.
• Concentration: 0.42 mg/ml (varies by lot)
• Sequence Length: 731

• Applicable Applications for anti-SMURF1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), IP, WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: SMURF1 (NP_065162, 165 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: SMURF1 antibody was raised against sMURF1 (NP_065162, 165 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SMURF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody, clone 1D7.)

Western Blot (WB)

(Western blot of SMURF1 expression in Raw 264.7 cells with SMURF1 monoclonal antibody, clone 1D7.)

Immunoprecipitation (IP)

(Immunoprecipitation of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody and Protein A Magnetic Bead.)

ELISA (EIA)

(Detection limit for recombinant GST tagged SMURF1 is approximately 0.1 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 31317290
• NCBI GeneID: 57154
• NCBI Accession #: NP_851994.1
• NCBI GenBank Nucleotide #: NM_181349.2
• UniProt Accession #: Q9HCE7; O75853; Q547Q3; Q9UJT8; A4D279; B7ZMB6; B9EGV3
• Molecular Weight: 83,440 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase SMURF1 isoform 2
• NCBI Official Synonym Full Names: SMAD specific E3 ubiquitin protein ligase 1
• NCBI Official Symbol: SMURF1
• NCBI Protein Information: E3 ubiquitin-protein ligase SMURF1; hSMURF1; E3 ubiquitin ligase SMURF1; Smad-specific E3 ubiquitin ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1
• UniProt Protein Name: E3 ubiquitin-protein ligase SMURF1
• UniProt Gene Name: SMURF1
• UniProt Synonym Gene Names: KIAA1625; hSMURF1
• UniProt Entry Name: SMUF1_HUMAN

NCBI Description

This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010]

Uniprot Description

SMURF1: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; cell soma; axon; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: protein binding; activin binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; ubiquitin-dependent SMAD protein catabolic process; negative regulation of BMP signaling pathway; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; receptor catabolic process; negative regulation of ossification; ectoderm development; protein export from nucleus; cell differentiation; negative regulation of transforming growth factor beta receptor signaling pathway